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Human DNA-binding protein Ikaros / IKZF1 ELISA Kit

SKU:
HUFI01303
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q13422
Sensitivity:
0.094ng/ml
Range:
0.156-10ng/ml
ELISA Type:
Sandwich
Synonyms:
IKZF1, DNA-binding protein Ikaros, Ikaros family zinc finger protein 1, Lymphoid transcription factor LyF-1, IK1, IKAROS, LYF1, PRO0758, ZNFN1A1, hIk-1, ZNFN1A1CLL-associated antigen KW-6
Reactivity:
Human
Research Area:
Cell Cycle
€599
Frequently bought together:

Description

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Human DNA-binding protein Ikaros / IKZF1 ELISA Kit

DNA-binding protein Ikaros/IKZF1 codes for a transcription factor that is part of the zinc-finger DNA-binding protein family that is involved in chromatin remodelling. DNA-binding protein Ikaros/IKZF1 is only found in the foetal and adult hemo-lymphopoietic systems, and it works as a lymphocyte differentiation regulator. The number of N-terminal zinc finger motifs that bind DNA and the presence of a nuclear localization signal change between isoforms, resulting in members with and without DNA-binding characteristics. Only a few isoforms have the three or more N-terminal zinc motifs required for high affinity binding to a particular core DNA sequence region in target gene promoters. B-cell cancers, such as acute lymphoblastic leukaemia, have been linked to overexpression of several dominant-negative isoforms. Immunodeficiency, Common Variable 13, and Diamond-Blackfan Anemia-Like are diseases linked to IKZF1.

Product Name:Human DNA-binding protein Ikaros / IKZF1 ELISA Kit
Product Code:HUFI01303
Size:96 Assays
Alias:IKZF1, DNA-binding protein Ikaros, Ikaros family zinc finger protein 1, Lymphoid transcription factor LyF-1, IK1, IKAROS, LYF1, PRO0758, ZNFN1A1, hIk-1, ZNFN1A1CLL-associated antigen KW-6
Detection method:Sandwich ELISA, Double Antibody
Application:This immunoassay kit allows for the in vitro quantitative determination of Human IKZF1 concentrations in serum plasma and other biological fluids.
Sensitivity:0.094ng/ml
Range:0.156-10ng/ml
Storage:4°C for 6 months
Note:For Research Use Only
Recovery:Matrices listed below were spiked with certain level of Human IKZF1 and the recovery rates were calculated by comparing the measured value to the expected amount of Human IKZF1 in samples.
MatrixRecovery range(%)Average(%)
serum(n=5)87-10598
EDTA plasma(n=5)90-9995
UFH plasma(n=5)87-10496
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human IKZF1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample1:21:41:8
serum(n=5)89-97%85-94%85-101%
EDTA plasma(n=5)82-94%82-100%83-96%
UFH plasma(n=5)82-96%80-87%83-98%
CV(%):Intra-Assay: CV<8%
Inter-Assay: CV<10%
ComponentQuantityStorage
ELISA Microplate (Dismountable)8×12 strips4°C for 6 months
Lyophilized Standard24°C/-20°C
Sample/Standard Dilution Buffer20ml4°C
Biotin-labeled Antibody(Concentrated)120ul4°C (Protect from light)
Antibody Dilution Buffer10ml4°C
HRP-Streptavidin Conjugate(SABC)120ul4°C (Protect from light)
SABC Dilution Buffer10ml4°C
TMB Substrate10ml4°C (Protect from light)
Stop Solution10ml4°C
Wash Buffer(25X)30ml4°C
Plate Sealer5-

Other materials and equipment required:

  • Microplate reader with 450 nm wavelength filter
  • Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
  • Incubator
  • Deionized or distilled water
  • Absorbent paper
  • Buffer resevoir
UniprotQ13422
UniProt Protein Function:Ikaros: a transcription factor of the ikaros C2H2-type zinc-finger protein family. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Functions in the specification and the maturation of the T-lymphocyte. Also interacts with a critical control element in the TDT (terminal deoxynucleotidyltransferase) promoter as well as with the promoters for other genes expressed during early stages of B- and T-cell development. Deletions in Ikaros have been observed in a subset of pre-B-cell acute lymphoblastic leukemia (B-ALL) cases. Seven alternatively spliced human isoforms have been described.
UniProt Protein Details:Protein type:C2H2-type zinc finger protein; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 7p12.2

Cellular Component: centric heterochromatin; protein complex; cytoplasm; nucleus

Molecular Function:DNA binding; sequence-specific DNA binding; protein heterodimerization activity; metal ion binding; transcription factor activity

Biological Process: Peyer's patch development; thymus development; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; positive regulation of multicellular organism growth; chromatin modification; cell cycle; lymph node development; positive regulation of neutrophil differentiation; retina development in camera-type eye; natural killer cell differentiation; B cell differentiation; forebrain development; positive regulation of NK T cell differentiation; mesoderm development; positive regulation of transcription from RNA polymerase II promoter; T cell differentiation

NCBI Summary:This gene encodes a transcription factor that belongs to the family of zinc-finger DNA-binding proteins associated with chromatin remodeling. The expression of this protein is restricted to the fetal and adult hemo-lymphopoietic system, and it functions as a regulator of lymphocyte differentiation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. Most isoforms share a common C-terminal domain, which contains two zinc finger motifs that are required for hetero- or homo-dimerization, and for interactions with other proteins. The isoforms, however, differ in the number of N-terminal zinc finger motifs that bind DNA and in nuclear localization signal presence, resulting in members with and without DNA-binding properties. Only a few isoforms contain the requisite three or more N-terminal zinc motifs that confer high affinity binding to a specific core DNA sequence element in the promoters of target genes. The non-DNA-binding isoforms are largely found in the cytoplasm, and are thought to function as dominant-negative factors. Overexpression of some dominant-negative isoforms have been associated with B-cell malignancies, such as acute lymphoblastic leukemia (ALL). [provided by RefSeq, May 2014]
UniProt Code:Q13422
NCBI GenInfo Identifier:3913926
NCBI Gene ID:10320
NCBI Accession:Q13422.1
UniProt Related Accession:Q13422
Molecular Weight:
NCBI Full Name:DNA-binding protein Ikaros
NCBI Synonym Full Names:IKAROS family zinc finger 1
NCBI Official Symbol:IKZF1  
NCBI Official Synonym Symbols:IK1; LYF1; LyF-1; CVID13; IKAROS; PPP1R92; PRO0758; ZNFN1A1; Hs.54452  
NCBI Protein Information:DNA-binding protein Ikaros
UniProt Protein Name:DNA-binding protein Ikaros
UniProt Synonym Protein Names:Ikaros family zinc finger protein 1; Lymphoid transcription factor LyF-1
Protein Family:Ikaros family zinc finger protein
UniProt Gene Name:IKZF1  
UniProt Entry Name:IKZF1_HUMAN

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

Before adding to wells, equilibrate the SABC working solution and TMB substrate for at least 30 min at 37°C. When diluting samples and reagents, they must be mixed completely and evenly. It is recommended to plot a standard curve for each test.

StepProtocol
1.Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample in duplicate. Wash plate 2 times before adding standard, sample and control (zero) wells!
2.Aliquot 0.1ml standard solutions into the standard wells.
3.Add 0.1 ml of Sample / Standard dilution buffer into the control (zero) well.
4.Add 0.1 ml of properly diluted sample ( Human serum, plasma, tissue homogenates and other biological fluids.) into test sample wells.
5.Seal the plate with a cover and incubate at 37 °C for 90 min.
6.Remove the cover and discard the plate content, clap the plate on the absorbent filter papers or other absorbent material. Do NOT let the wells completely dry at any time. Wash plate X2.
7.Add 0.1 ml of Biotin- detection antibody working solution into the above wells (standard, test sample & zero wells). Add the solution at the bottom of each well without touching the side wall.
8.Seal the plate with a cover and incubate at 37°C for 60 min.
9.Remove the cover, and wash plate 3 times with Wash buffer. Let wash buffer rest in wells for 1 min between each wash.
10.Add 0.1 ml of SABC working solution into each well, cover the plate and incubate at 37°C for 30 min.
11.Remove the cover and wash plate 5 times with Wash buffer, and each time let the wash buffer stay in the wells for 1-2 min.
12.Add 90 µl of TMB substrate into each well, cover the plate and incubate at 37°C in dark within 10-20 min. (Note: This incubation time is for reference use only, the optimal time should be determined by end user.) And the shades of blue can be seen in the first 3-4 wells (with most concentrated standard solutions), the other wells show no obvious color.
13.Add 50 µl of Stop solution into each well and mix thoroughly. The color changes into yellow immediately.
14. Read the O.D. absorbance at 450 nm in a microplate reader immediately after adding the stop solution.

When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.

Sample TypeProtocol
SerumIf using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

PlasmaCollect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit.
Urine & Cerebrospinal FluidCollect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid.
Cell culture supernatantCollect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately.
Cell lysatesSolubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Tissue homogenatesThe preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C.
Tissue lysatesRinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Breast MilkCollect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles.

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