The Human Dickkopf Related Protein 3 (DKK3) ELISA Kit is designed to quantitatively measure DKK3 levels in a variety of human biological samples. DKK3, a member of the Dickkopf family, is known for its role in modulating the Wnt signaling pathway, which regulates cell differentiation, proliferation, and apoptosis. DKK3 has been implicated in various biological processes, including tumorigenesis, development, and tissue injury response, making it a crucial target of study in research and clinical applications.
Our DKK3 ELISA Kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results critical for unraveling the involvement of DKK3 in disease mechanisms. Manufactured under stringent quality control standards, this kit guarantees robust performance and user-friendly operation, making it an indispensable tool for your scientific investigations. Trust Assay Genie's DKK3 ELISA Kit for precise quantification of this important biomarker in your research studies.
Product Name:
Human DKK3 (Dickkopf Related Protein 3) ELISA Kit
SKU:
AEES00142
Target:
Human DKK3 (Dickkopf Related Protein 3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human DKK3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DKK3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DKK3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human DKK3. You can calculate the concentration of Human DKK3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-110
95-102
98-105
Average (%)
103
99
102
1:4
Range (%)
92-104
93-100
88-102
Average (%)
96
96
96
1:8
Range (%)
92-104
95-105
98-110
Average (%)
96
99
102
1:16
Range (%)
96-109
95-108
97-110
Average (%)
104
103
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-99
95
EDTA plasma (n=5)
94-106
102
Cell culture media (n=5)
93-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.21
5.61
22.62
2.39
5.96
24.62
Standard deviation
0.13
0.26
1.15
0.13
0.28
1.35
C V (%)
5.88
4.63
5.08
5.44
4.7
5.48
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human DKK3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human DKK3 in samples. No significant cross-reactivity or interference between Human DKK3 and analogues was observed.
