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Human Dipeptidyl Peptidase IV (DPP4) ELISA Kit

SKU:
HUDL00865
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P27487
Sensitivity:
14.8pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
CD26,ADA,DPPIV,DPP-IV,ADABP,ADCP2,TP103,Adenosine Deaminase Complexing Protein 2,T-cell activation antigen CD26
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

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Human Dipeptidyl Peptidase IV (DPP4) ELISA Kit

The Human Dipeptidyl Peptidase IV (DPP4) ELISA Kit is a reliable tool for the quantitative measurement of DPP4 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results across a variety of research applications.Dipeptidyl Peptidase IV (DPP4) is an important enzyme involved in the regulation of glucose metabolism and immune function. Dysregulation of DPP4 activity has been linked to conditions such as diabetes, inflammation, and cancer, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.

This ELISA kit provides researchers with a precise and efficient method for assessing DPP4 levels in biological samples, allowing for in-depth investigations into the role of this enzyme in health and disease. Trust in the Human Dipeptidyl Peptidase IV (DPP4) ELISA Kit for accurate and insightful research outcomes.

Product Name:Human Dipeptidyl Peptidase IV (DPP4) ELISA Kit
Product Code:HUDL00865
Size:96 Assays
Target:DPP4
Synonyms:CD26, ADA, DPPIV, DPP-IV, ADABP, ADCP2, TP103, Adenosine Deaminase Complexing Protein 2, T-cell activation antigen CD26
Detection Method:Sandwich
Range:31.25-2000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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