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Human Dihydrolipoyl Dehydrogenase (DLD) ELISA Kit

SKU:
HUDL00836
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P09622
Sensitivity:
1.3U/L
Range:
1.56-100U/L
ELISA Type:
Sandwich
Synonyms:
LDH-D,DLD,Probable D-lactate dehydrogenase, mitochondrial
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Dihydrolipoyl Dehydrogenase (DLD) ELISA Kit

The Human Dihydrolipoyl Dehydrogenase (DLD) ELISA Kit is a highly reliable and accurate tool for measuring DLD levels in human samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers precise and reproducible results, making it suitable for a variety of research applications.DLD is an essential enzyme involved in the tricarboxylic acid cycle and oxidative phosphorylation, playing a critical role in cellular energy production.

Dysregulation of DLD activity has been linked to various metabolic disorders and mitochondrial diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.Whether investigating mitochondrial function, metabolic diseases, or related research areas, the Human DLD ELISA Kit provides a valuable tool for accurately measuring DLD levels and advancing scientific knowledge in these fields.

Product Name:Human Dihydrolipoyl Dehydrogenase (DLD) ELISA Kit
Product Code:HUDL00836
Size:96 Assays
Target:DLD
Synonyms:LDH-D, DLD, Probable D-lactate dehydrogenase, mitochondrial
Detection Method:Sandwich
Range:1.56-100U/L
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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