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Human DHRS4 Recombinant Protein (RPPB1574)

SKU:
RPPB1574
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9BTZ2
Research Area:
Enzymes
€309
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Description

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Product Name:Human DHRS4 Recombinant Protein
Product Code:RPPB1574
Size:5µg
Species:Human
Target:DHRS4
Synonyms:Dehydrogenase/reductase SDR family member 4, NADPH-dependent carbonyl reductase/NADP-retinol dehydrogenase, CR, PHCR, NADPH-dependent retinol dehydrogenase/reductase, NRDR, humNRDR, Peroxisomal short-chain alcohol dehydrogenase, PSCD, SCAD-SRL, Short-chain dehydrogenase/reductase family member 4, DHRS4, SDR-SRL, SDR25C1, SDR25C2.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The DHRS4 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH7.5), 20% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSHMHKAGL LGLCARAWNS VRMASSGMTR RDPLANKVAL VTASTDGIGF AIARRLAQDG AHVVVSSRKQ QNVDQAVATL QGEGLSVTGT VCHVGKAEDR ERLVATAVKL HGGIDILVSN AAVNPFFGSI MDVTEEVWDK TLDINVKAPA LMTKAVVPEM EKRGGGSVVI VSSIAAFSPS PGFSPYNVSK TALLGLTKTL AIELAPRNIR VNCLAPGLIK TSFSRMLWMD KEKEESMKET LRIRRLGEPE DCAGIVSFLC SEDASYITGE TVVVGGGTPS RL

Dehydrogenase/reductase SDR family member 4 (DHRS4) is a member of the short-chain dehydrogenases/reductases (SDR) family. DHRS4 reduces all trans retinal and 9-cis retinal. In addition, the DHRS4 protein can catalyze the oxidation of all trans retinol with NADP as cofactor, but with a much lower efficiency. Furthermore, DHRS4 reduces alkyl phenyl ketones and alpha dicarbonyl compounds with aromatic rings, such as pyrimidine 4 aldehyde, 3 benzoylpyridine, 4 benzoylpyridine, menadione and 4 hexanoylpyridine.

DHRS4 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 302 amino acids (1-278) and having a molecular mass of 32.1kDa.DHRS4 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:DHRS4: Reduces all-trans-retinal and 9-cis retinal. Can also catalyze the oxidation of all-trans-retinol with NADP as co- factor, but with much lower efficiency. Reduces alkyl phenyl ketones and alpha-dicarbonyl compounds with aromatic rings, such as pyrimidine-4-aldehyde, 3-benzoylpyridine, 4-benzoylpyridine, menadione and 4-hexanoylpyridine. Has no activity towards aliphatic aldehydes and ketones. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 7 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Oxidoreductase; EC 1.1.1.184; Cofactor and Vitamin Metabolism - retinol

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: peroxisomal membrane; endoplasmic reticulum membrane; nuclear membrane; mitochondrion; intracellular membrane-bound organelle; peroxisome; nucleus

Molecular Function:oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; alcohol dehydrogenase [NAD(P)+] activity; 3-keto sterol reductase activity; carbonyl reductase (NADPH) activity; receptor binding

Biological Process: steroid metabolic process; ketone metabolic process; alcohol metabolic process; protein tetramerization

UniProt Code:Q9BTZ2
NCBI GenInfo Identifier:308153604
NCBI Gene ID:10901
NCBI Accession:Q9BTZ2.3
UniProt Secondary Accession:Q9BTZ2,O95162, Q20CR0, Q2LC19, Q2LE81, Q58IU4, Q6E0Y1 Q6UWU3, B2RB10, B7WNS9, D3YTB8, E2QRL8,
UniProt Related Accession:Q9BTZ2
Molecular Weight:23,917 Da
NCBI Full Name:Dehydrogenase/reductase SDR family member 4
NCBI Synonym Full Names:dehydrogenase/reductase (SDR family) member 4
NCBI Official Symbol:DHRS4��
NCBI Official Synonym Symbols:CR; NRDR; PHCR; PSCD; SDR-SRL; SDR25C1; SDR25C2; SCAD-SRL��
NCBI Protein Information:dehydrogenase/reductase SDR family member 4; peroxisomal short-chain alcohol dehydrogenase; NADPH-dependent retinol dehydrogenase/reductase; short-chain dehydrogenase/reductase family member 4; dehydrogenase/reductase (SDR family) member 4 like 2A; short chain dehydrogenase/reductase family 25C, member 1; short chain dehydrogenase/reductase family 25C, member 2; NADPH-dependent carbonyl reductase/NADP-retinol dehydrogenase
UniProt Protein Name:Dehydrogenase/reductase SDR family member 4
UniProt Synonym Protein Names:NADPH-dependent carbonyl reductase/NADP-retinol dehydrogenase; CR; PHCR; NADPH-dependent retinol dehydrogenase/reductase; NRDR; humNRDR; Peroxisomal short-chain alcohol dehydrogenase; PSCD; SCAD-SRL; Short-chain dehydrogenase/reductase family member 4
UniProt Gene Name:DHRS4��
UniProt Entry Name:DHRS4_HUMAN

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