Human DHH Recombinant Protein (RPPB0907)
- SKU:
- RPPB0907
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- O43323
- Research Area:
- Cytokines
Description
| Product Name: | Human DHH Recombinant Protein |
| Product Code: | RPPB0907 |
| Size: | 25µg |
| Species: | Human |
| Target: | DHH |
| Synonyms: | HHG-3, Desert Hedgehog homolog, MGC35145, Desert hedgehog protein, DHH. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | DHH solution containing 20mM MES pH-5.5, 0.5mM DTT and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MCGPGRGPVG RRRYARKQLV PLLYKQFVPG VPERTLGASG PAEGRVARGS ERFRDLVPNY NPDIIFKDEE NSGADRLMTE RCKERVNALA IAVMNMWPGV RLRVTEGWDE DGHHAQDSLH YEGRALDITT SDRDRNKYGL LARLAVEAGF DWVYYESRNH VHVSVKADNS LAVRAGG |
DHH is part of the Hedgehog family which encodes signaling molecules that are involved in regulating morphogenesis. DHH protein is a precursor that is autocatalytically cleaved, the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. Additionally, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the organism. Defects in DHH protein have been associated with partial gonadal dysgenesis (PGD) accompanied by minifascicular polyneuropathy. DHH plays a role both male gonadal differentiation and perineurial development.DHH plays a role in intercellular signaling which is essential for a variety of patterning events during development. DHH functions as a spermatocyte survival factor in the testes & is essential for testes development.
DHH Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 197 amino acids (23-198) and having a molecular mass of 22 kDa. DHH is fused to His-tag (20 a.a.) at N-terminus and is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | DHH: Intercellular signal essential for a variety of patterning events during development. May function as a spermatocyte survival factor in the testes. Essential for testes development. Defects in DHH may be the cause of partial gonadal dysgenesis with minifascicular neuropathy 46,XY (PGD). PGD is characterized by the presence of a testis on one side and a streak or an absent gonad at the other, persistence of Muellerian duct structures, and a variable degree of genital ambiguity. Defects in DHH may be the cause of complete pure gonadal dysgenesis 46,XY type (GDXYM); also known as male- limited gonadal dysgenesis 46,XY. GDXYM is a type of hypogonadism in which no functional gonads are present to induce puberty in an externally female person whose karyotype is then found to be XY. The gonads are found to be non-functional streaks. Belongs to the hedgehog family. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide; Cell development/differentiation Chromosomal Location of Human Ortholog: 12q13.1 Cellular Component: extracellular space; plasma membrane Molecular Function:peptidase activity; protein binding; zinc ion binding; patched binding; calcium ion binding Biological Process: myelination; Leydig cell differentiation; cell-cell signaling; male sex determination; regulation of steroid biosynthetic process; proteolysis; spermatid development; response to estradiol stimulus Disease: 46,xy Gonadal Dysgenesis, Partial, With Minifascicular Neuropathy; 46,xy Sex Reversal 7 |
| NCBI Summary: | This gene encodes a member of the hedgehog family. The hedgehog gene family encodes signaling molecules that play an important role in regulating morphogenesis. This protein is predicted to be made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the organism. Defects in this protein have been associated with partial gonadal dysgenesis (PGD) accompanied by minifascicular polyneuropathy. This protein may be involved in both male gonadal differentiation and perineurial development. [provided by RefSeq, May 2010] |
| UniProt Code: | O43323 |
| NCBI GenInfo Identifier: | 6166118 |
| NCBI Gene ID: | 50846 |
| NCBI Accession: | O43323.1 |
| UniProt Secondary Accession: | O43323,Q15794, |
| UniProt Related Accession: | O43323 |
| Molecular Weight: | 43,577 Da |
| NCBI Full Name: | Desert hedgehog protein |
| NCBI Synonym Full Names: | desert hedgehog |
| NCBI Official Symbol: | DHH�� |
| NCBI Official Synonym Symbols: | GDXYM; HHG-3; SRXY7�� |
| NCBI Protein Information: | desert hedgehog protein; mutant desert hedgehog; desert hedgehog homolog |
| UniProt Protein Name: | Desert hedgehog protein |
| UniProt Synonym Protein Names: | HHG-3 |
| Protein Family: | Desert hedgehog protein |
| UniProt Gene Name: | DHH�� |
| UniProt Entry Name: | DHH_HUMAN |
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