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Human Dermcidin (DCD) ELISA Kit

SKU:
HUDL00795
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P81605
Sensitivity:
0.31ng/mL
Range:
0.781-50ng/mL
ELISA Type:
Sandwich
Synonyms:
HCAP,AIDD,DCD1,DSEP,PIF,Proteolysis Inducing Factor,Preproteolysin,Diffusible Survival/Evasion Peptide,Survival Promoting Peptide
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Human Dermcidin (DCD) ELISA Kit

The Human Dermcidin (DCD) ELISA Kit is specifically designed for the sensitive and precise detection of Dermcidin levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional accuracy and consistency, ensuring dependable results for a variety of research purposes.Dermcidin is a key antimicrobial peptide that plays a vital role in the innate immune response, defending against pathogens and maintaining skin health. Abnormal levels of Dermcidin have been linked to various skin disorders and infections, emphasizing its significance as a biomarker in dermatological research and clinical studies.

With its high performance and versatility, the Human Dermcidin (DCD) ELISA Kit is a valuable tool for researchers investigating the role of Dermcidin in skin health, immune function, and disease pathogenesis. Order now and unlock the potential of studying Dermcidin in your research projects.

Product Name:Human Dermcidin (DCD) ELISA Kit
Product Code:HUDL00795
Size:96 Assays
Target:DCD
Synonyms:HCAP, AIDD, DCD1, DSEP, PIF, Proteolysis Inducing Factor, Preproteolysin, Diffusible Survival/Evasion Peptide, Survival Promoting Peptide
Detection Method:Sandwich
Range:0.781-50ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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