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Human Deoxyribonuclease X (DNASEX) ELISA Kit

SKU:
HUDL00851
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P49184
Sensitivity:
0.057ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
DNASE1L1,DNAS1L1,XIB,DNase-X,DNL1L,Deoxyribonuclease I-Like 1,Muscle-specific DNase I-like
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Human Deoxyribonuclease X (DNASEX) ELISA Kit

The Human Deoxyribonuclease X (DNaseX) ELISA Kit is specifically designed for the accurate quantification of DNaseX levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for various research applications.DNaseX is an essential enzyme that plays a critical role in DNA metabolism and cell survival by degrading DNA molecules. Dysregulation of DNaseX has been linked to various diseases, making it a valuable biomarker for studying conditions such as autoimmune disorders, cancer, and inflammatory diseases.

With its advanced technology and user-friendly protocol, the Human DNaseX ELISA Kit is a powerful tool for researchers seeking to investigate the role of DNaseX in disease pathogenesis and potential therapeutic interventions. Discover new insights with confidence using this reliable and innovative ELISA kit.

Product Name:Human Deoxyribonuclease X (DNASEX) ELISA Kit
Product Code:HUDL00851
Size:96 Assays
Target:DNASEX
Synonyms:DNASE1L1, DNAS1L1, XIB, DNase-X, DNL1L, Deoxyribonuclease I-Like 1, Muscle-specific DNase I-like
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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