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Human Defensin Alpha 1, Neutrophil (DEFa1) ELISA Kit

SKU:
HUDL00809
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P59665
Sensitivity:
0.146ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
DEF1,DEFA2,HNP-1,HP-1,MRS,DEF3,HNP-3,HNP3,HP-3,Defensin,Alpha 1,Myeloid-Related Sequence
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

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Human Defensin Alpha 1, Neutrophil (DEFa1) ELISA Kit

The Human Defensin Alpha 1 Neutrophil (DEFA1) ELISA Kit is a powerful tool for the precise measurement of DEFA1 levels in human samples such as serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it suitable for a variety of research purposes.DEFA1 is a key component of the body's innate immune response, playing a critical role in defending against microbial infections.

Studies have shown that abnormalities in DEFA1 levels are associated with various diseases, including inflammatory disorders and autoimmune conditions. As such, the DEFA1 ELISA Kit is essential for investigating these diseases and exploring potential therapeutic strategies.Get reliable and insightful data on DEFA1 with the Human Defensin Alpha 1 Neutrophil ELISA Kit, and advance your research in immunology, infectious diseases, and beyond.

Product Name:Human Defensin Alpha 1, Neutrophil (DEFa1) ELISA Kit
Product Code:HUDL00809
Size:96 Assays
Target:DEFa1
Synonyms:DEF1, DEFA2, HNP-1, HP-1, MRS, DEF3, HNP-3, HNP3, HP-3, Defensin,Alpha 1, Myeloid-Related Sequence
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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