The Human DEFA5 (Defensin Alpha 5) ELISA Kit is specifically designed for the accurate measurement of DEFA5 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring robust and consistent results for a variety of research applications.Defensin Alpha 5 is a paneth cell-specific protein that plays a crucial role in the immune response, defending against microbial infections and maintaining intestinal homeostasis. Dysregulation of DEFA5 has been implicated in inflammatory bowel diseases, autoimmune disorders, and other pathological conditions, making it a valuable biomarker for studying these diseases and developing potential therapeutic interventions.
With its user-friendly design and reliable performance, the Human DEFA5 ELISA Kit is a valuable tool for researchers in immunology, microbiology, and molecular biology seeking to explore the role of Defensin Alpha 5 in health and disease. Visit the link for more information: www.assaygenie.com/human-def-alpha5-defensin-alpha-5-paneth-cell-specific-elisa-kit-hues02764/
Human DEF alpha5 (Defensin Alpha 5, Paneth Cell Specific)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human DEFα5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DEFα5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DEFα5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human DEFα5. You can calculate the concentration of Human DEFα5 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-101
97-113
85-97
Average (%)
94
104
91
1:4
Range (%)
87-100
84-93
88-101
Average (%)
93
89
94
1:8
Range (%)
91-104
82-92
88-102
Average (%)
96
87
95
1:16
Range (%)
94-109
81-94
88-99
Average (%)
100
87
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-105
99
EDTA plasma (n=5)
90-104
97
Cell culture media (n=5)
87-99
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.38
92.81
486.57
45.03
99.71
477.42
Standard deviation
2.72
5.1
25.25
2.48
5.71
24.92
C V (%)
5.62
5.5
5.19
5.51
5.73
5.22
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human DEFα5 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human DEFα5 in samples. No significant cross-reactivity or interference between Human DEFα5 and analogues was observed.
