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Human DDR2 / Discoidin domain-containing receptor 2 ELISA Kit

SKU:
HUFI01445
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q16832
Sensitivity:
0.094ng/ml
Range:
0.156-10ng/ml
ELISA Type:
Sandwich
Synonyms:
DDR2, Discoidin domain-containing receptor 2, Discoidin domain receptor 2, Tyrosine-protein kinase TYRO10, Receptor protein-tyrosine kinase TKT, Neurotrophic tyrosine kinase, receptor-related 3, CD167 antigen-like family member B, Discoidin domain-co
Reactivity:
Human
Research Area:
Cell Biology
€599
Frequently bought together:

Description

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Human DDR2/Discoidin domain-containing receptor 2 ELISA Kit

The Human DDR2 (Discoidin Domain-Containing Receptor 2) ELISA Kit is specifically designed for the accurate detection of DDR2 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures reliable and reproducible results, making it an ideal tool for researchers in various fields.DDR2 is a cell surface receptor involved in cell adhesion and migration, as well as collagen binding and activation.

Dysregulation of DDR2 expression has been linked to various diseases, including cancer, fibrosis, and osteoarthritis, making it a critical biomarker for understanding these conditions and developing targeted therapies.With the Human DDR2 ELISA Kit, researchers can confidently study the role of DDR2 in disease progression and therapeutic development, ultimately advancing our understanding of its impact on human health.

Product Name:Human DDR2 / Discoidin domain-containing receptor 2 ELISA Kit
Product Code:HUFI01445
Size:96 Assays
Alias:DDR2, Discoidin domain-containing receptor 2, Discoidin domain receptor 2, Tyrosine-protein kinase TYRO10, Receptor protein-tyrosine kinase TKT, Neurotrophic tyrosine kinase, receptor-related 3, CD167 antigen-like family member B, Discoidin domain-containing receptor tyrosine kinase 2, NTRKR3, TKT, TYRO10, Trk3, CD167b antigen
Detection method:Sandwich ELISA, Double Antibody
Application:This immunoassay kit allows for the in vitro quantitative determination of Human DDR2 concentrations in serum plasma and other biological fluids.
Sensitivity:0.094ng/ml
Range:0.156-10ng/ml
Storage:4°C for 6 months
Note:For Research Use Only
Recovery:Matrices listed below were spiked with certain level of Human DDR2 and the recovery rates were calculated by comparing the measured value to the expected amount of Human DDR2 in samples.
MatrixRecovery range(%)Average(%)
serum(n=5)96-103100
EDTA plasma(n=5)85-10092
UFH plasma(n=5)85-10196
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human DDR2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample1:21:41:8
serum(n=5)85-105%90-100%87-102%
EDTA plasma(n=5)85-99%83-91%84-100%
UFH plasma(n=5)84-96%84-98%81-100%
CV(%):Intra-Assay: CV<8%
Inter-Assay: CV<10%
ComponentQuantityStorage
ELISA Microplate (Dismountable)8×12 strips4°C for 6 months
Lyophilized Standard24°C/-20°C
Sample/Standard Dilution Buffer20ml4°C
Biotin-labeled Antibody(Concentrated)120ul4°C (Protect from light)
Antibody Dilution Buffer10ml4°C
HRP-Streptavidin Conjugate(SABC)120ul4°C (Protect from light)
SABC Dilution Buffer10ml4°C
TMB Substrate10ml4°C (Protect from light)
Stop Solution10ml4°C
Wash Buffer(25X)30ml4°C
Plate Sealer5-

Other materials and equipment required:

  • Microplate reader with 450 nm wavelength filter
  • Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
  • Incubator
  • Deionized or distilled water
  • Absorbent paper
  • Buffer resevoir
UniprotQ16832
UniProt Protein Function:DDR2: This tyrosine kinase receptor for fibrillar collagen mediates fibroblast migration and proliferation. Contributes to cutaneous wound healing. Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
UniProt Protein Details:Protein type:Membrane protein, integral; EC 2.7.10.1; Protein kinase, TK; Protein kinase, tyrosine (receptor); Kinase, protein; TK group; DDR family

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: focal adhesion; integral to plasma membrane; apical plasma membrane; plasma membrane

Molecular Function:collagen binding; protein binding; transmembrane receptor protein tyrosine kinase activity; ATP binding

Biological Process: extracellular matrix organization and biogenesis; ossification; peptidyl-tyrosine phosphorylation; collagen fibril organization; protein amino acid autophosphorylation; signal transduction; positive regulation of osteoblast differentiation; positive regulation of fibroblast proliferation; biomineral formation; positive regulation of protein kinase activity; positive regulation of transcription factor activity; cell adhesion; regulation of bone mineralization

Disease: Spondylometaepiphyseal Dysplasia, Short Limb-hand Type

NCBI Summary:This gene encodes a member of the discoidin domain receptor subclass of the receptor tyrosine kinase (RTKs) protein family. RTKs play a key role in the communication of cells with their microenvironment. The encoded protein is a collagen-induced receptor that activates signal transduction pathways involved in cell adhesion, proliferation, and extracellular matrix remodeling. This protein is expressed in numerous cell types and may alos be involved in wound repair and regulate tumor growth and invasiveness. Mutations in this gene are the cause of short limb-hand type spondylometaepiphyseal dysplasia. [provided by RefSeq, Aug 2017]
UniProt Code:Q16832
NCBI GenInfo Identifier:215273969
NCBI Gene ID:4921
NCBI Accession:Q16832.2
UniProt Related Accession:Q16832
Molecular Weight:
NCBI Full Name:Discoidin domain-containing receptor 2
NCBI Synonym Full Names:discoidin domain receptor tyrosine kinase 2
NCBI Official Symbol:DDR2  
NCBI Official Synonym Symbols:TKT; WRCN; MIG20a; NTRKR3; TYRO10  
NCBI Protein Information:discoidin domain-containing receptor 2
UniProt Protein Name:Discoidin domain-containing receptor 2
UniProt Synonym Protein Names:CD167 antigen-like family member B; Discoidin domain-containing receptor tyrosine kinase 2; Neurotrophic tyrosine kinase, receptor-related 3; Receptor protein-tyrosine kinase TKT; Tyrosine-protein kinase TYRO10; CD_antigen: CD167b
Protein Family:Discoidin domain-containing receptor
UniProt Gene Name:DDR2  
UniProt Entry Name:DDR2_HUMAN

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

Before adding to wells, equilibrate the SABC working solution and TMB substrate for at least 30 min at 37°C. When diluting samples and reagents, they must be mixed completely and evenly. It is recommended to plot a standard curve for each test.

StepProtocol
1.Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample in duplicate. Wash plate 2 times before adding standard, sample and control (zero) wells!
2.Aliquot 0.1ml standard solutions into the standard wells.
3.Add 0.1 ml of Sample / Standard dilution buffer into the control (zero) well.
4.Add 0.1 ml of properly diluted sample ( Human serum, plasma, tissue homogenates and other biological fluids.) into test sample wells.
5.Seal the plate with a cover and incubate at 37 °C for 90 min.
6.Remove the cover and discard the plate content, clap the plate on the absorbent filter papers or other absorbent material. Do NOT let the wells completely dry at any time. Wash plate X2.
7.Add 0.1 ml of Biotin- detection antibody working solution into the above wells (standard, test sample & zero wells). Add the solution at the bottom of each well without touching the side wall.
8.Seal the plate with a cover and incubate at 37°C for 60 min.
9.Remove the cover, and wash plate 3 times with Wash buffer. Let wash buffer rest in wells for 1 min between each wash.
10.Add 0.1 ml of SABC working solution into each well, cover the plate and incubate at 37°C for 30 min.
11.Remove the cover and wash plate 5 times with Wash buffer, and each time let the wash buffer stay in the wells for 1-2 min.
12.Add 90 µl of TMB substrate into each well, cover the plate and incubate at 37°C in dark within 10-20 min. (Note: This incubation time is for reference use only, the optimal time should be determined by end user.) And the shades of blue can be seen in the first 3-4 wells (with most concentrated standard solutions), the other wells show no obvious color.
13.Add 50 µl of Stop solution into each well and mix thoroughly. The color changes into yellow immediately.
14. Read the O.D. absorbance at 450 nm in a microplate reader immediately after adding the stop solution.

When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.

Sample TypeProtocol
SerumIf using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

PlasmaCollect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit.
Urine & Cerebrospinal FluidCollect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid.
Cell culture supernatantCollect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately.
Cell lysatesSolubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Tissue homogenatesThe preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C.
Tissue lysatesRinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Breast MilkCollect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles.

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