Human DDIT4 Recombinant Protein (RPPB3344)
- SKU:
- RPPB3344
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q9NX09
Description
| Product Name: | Human DDIT4 Recombinant Protein |
| Product Code: | RPPB3344 |
| Size: | 10µg |
| Species: | Human |
| Target: | DDIT4 |
| Synonyms: | DNA-damage-inducible transcript 4, RTP801, REDD-1, Dig2, FLJ20500, Protein regulated in development and DNA damage response 1, HIF-1 responsive protein RTP801, RP11-442H21.1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The DDIT4 protein solution (0.25mg/1ml) is formulated in 20 mM Tris-HCl buffer (pH8.0), 0.2M NaCl, 5mM DTT, 1mM EDTA and 30% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Please avoid freeze thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MPSLWDRFSS SSTSSSPSSL PRTPTPDRPP RSAWGSATRE EGFDRSTSLE SSDCESLDSS NSGFGPEEDT AYLDGVSLPD FELLSDPEDE HLCANLMQLL QESLAQARLG SRRPARLLMP SQLVSQVGKE LLRLAYSEPC GLRGALLDVC VEQGKSCHSV GQLALDPSLV PTFQLTLVLR LDSRLWPKIQ GLFSSANSPF LPGFSQSLTL STGFRVIKKK LYSSEQLLIE EC |
DDIT4 has a role in the regulation of reactive oxygen species. DDIT4 is upregulated at the transcriptional level as a reaction to stress due to DNA damage and glucocorticoid treatment. DDIT4 negatively regulates the mammalian target of Rapamycin, a serine/threonine kinase frequently referred to as mTOR. DDIT4 is vital in the coupling of extra- and intracellular cues to mTOR regulation.
DDIT4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 252 amino acids (1-232.a.a) and having a molecular mass of 27.5kDa. DDIT4 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | DDIT4: Inhibits cell growth by regulating the TOR signaling pathway upstream of the TSC1-TSC2 complex and downstream of AKT1. Promotes neuronal cell death. Up-regulated in fibroblasts upon ionizing radiation, via a TP53-dependent pathway. Up-regulated by TP63 in primary keratinocytes, and down-regulated during keratinocyte differentiation. Up-regulated upon DNA alkylation. Up-regulated by amyloid beta-peptide and retinoic acid. Up-regulated by hypoxia, via a PI3K and HIF1A-dependent but TP53/TP63-independent mechanism. Broadly expressed, with lowest levels in brain, skeletal muscle and intestine. Up-regulated in substantia nigra neurons from Parkinson disease patients. Belongs to the DDIT4 family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 10q22.1 Cellular Component: cytoplasm; cytosol; intracellular Biological Process: brain development; cell proliferation; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; negative regulation of peptidyl-serine phosphorylation; negative regulation of TOR signaling pathway; nerve growth factor receptor signaling pathway; neuron differentiation; neuron migration; response to hypoxia |
| UniProt Code: | Q9NX09 |
| NCBI GenInfo Identifier: | 74753036 |
| NCBI Gene ID: | 54541 |
| NCBI Accession: | Q9NX09.1 |
| UniProt Secondary Accession: | Q9NX09,Q9H0S3, |
| UniProt Related Accession: | Q9NX09 |
| Molecular Weight: | 25,371 Da |
| NCBI Full Name: | DNA damage-inducible transcript 4 protein |
| NCBI Synonym Full Names: | DNA damage inducible transcript 4 |
| NCBI Official Symbol: | DDIT4�� |
| NCBI Official Synonym Symbols: | Dig2; REDD1; REDD-1�� |
| NCBI Protein Information: | DNA damage-inducible transcript 4 protein |
| UniProt Protein Name: | DNA damage-inducible transcript 4 protein |
| UniProt Synonym Protein Names: | HIF-1 responsive protein RTP801; Protein regulated in development and DNA damage response 1; REDD-1 |
| Protein Family: | DNA damage-inducible transcript 4 protein |
| UniProt Gene Name: | DDIT4�� |
| UniProt Entry Name: | DDIT4_HUMAN |
