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Human DDIT3 Recombinant Protein (RPPB3343)

SKU:
RPPB3343
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P35638
€309
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Description

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Product Name:Human DDIT3 Recombinant Protein
Product Code:RPPB3343
Size:20µg
Species:Human
Target:DDIT3
Synonyms:DNA damage-inducible transcript 3, DDIT-3, Growth arrest and DNA-damage-inducible protein GADD153, C/EBP-homologous protein, CHOP, DDIT3, GADD153, CEBPZ, CHOP10, MGC4154.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The DDIT3 protein solution (1mg/ml) contains 20mM Tris-HCl pH-8 and 20% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MAAESLPFSF GTLSSWELEA WYEDLQEVLS SDENGGTYVS PPGNEEEESK IFTTLDPASL AWLTEEEPEP AEVTSTSQSP HSPDSSQSSL AQEEEEEDQG RTRKRKQSGH SPARAGKQRM KEKEQENERK VAQLAEENER LKQEIERLTR EVEATRRALI DRMVNLHQA

DDIT3 reduces DNA-binding activity of C/EBP and lap by forming heterodimers which don�t bind DNA. DDIT3, also known as GADD153, is a basic domain-leucine zipper(bZIP) transcription factor of C/EBP family. DDIT3 protein is up-regulated by several stresses, such as amino acid or glucose starvation, endoplasmic reticulum (ER) stress, osmotic stress and hypoxia. DDIT3 protein is invloved in ER stress-mediated apoptosis and in disease including diabetes, brain ischemia and neurodegenerative disease. DDIT3 plays a role in asoprisnil-induced apoptosis. Hypoglycaemia-induced necrotic cell death of neuroblastoma cells is an active process mediated via the induction of the transcription factor DDIT3. DDIT3 plays an important role in melanoma progression. HRG stimulation of mammary epithelial cells induces the expression of DDIT3 mRNA and protein and transcription of DDIT3 promoter.

DDIT3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 189 amino acids and having a molecular mass of 21 kDa. The DDIT3 protein is fused to a 20 amino acids His tag at N-terminus.The DDIT3 is purified by proprietary chromatographic techniques.

UniProt Protein Function:CHOP: a transcriptional-regulatory protein of the bZIP family. Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA. May play an important role in melanoma progression. CK2-mediated phosphorylation inhibits its transcriptional activity. Up-regulates IL-6 transcription by trapping negative regulating NF-IL6 isoform.
UniProt Protein Details:

Protein type:Autophagy; Oncoprotein; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 12q13.1-q13.2

Cellular Component: cytosol; nucleoplasm; nucleus

Molecular Function:cAMP response element binding protein binding; DNA binding; leucine zipper domain binding; protein binding; protein heterodimerization activity; protein homodimerization activity; transcription corepressor activity; transcription factor activity; transcription factor binding

Biological Process: cell cycle arrest; cell redox homeostasis; inhibition of CREB transcription factor; mRNA transcription from RNA polymerase II promoter; negative regulation of protein kinase B signaling cascade; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of interleukin-8 production; positive regulation of neuron apoptosis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; proteasomal ubiquitin-dependent protein catabolic process; regulation of transcription in response to stress; regulation of transcription, DNA-dependent; response to DNA damage stimulus; response to unfolded protein

Disease: Myxoid Liposarcoma

NCBI Summary:This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq, Aug 2010]
UniProt Code:P35638
NCBI GenInfo Identifier:544022
NCBI Gene ID:1649
NCBI Accession:P35638.1
UniProt Secondary Accession:P35638,F8VS99,
UniProt Related Accession:P35638
Molecular Weight:21,700 Da
NCBI Full Name:DNA damage-inducible transcript 3 protein
NCBI Synonym Full Names:DNA damage inducible transcript 3
NCBI Official Symbol:DDIT3��
NCBI Official Synonym Symbols:CHOP; CEBPZ; CHOP10; CHOP-10; GADD153��
NCBI Protein Information:DNA damage-inducible transcript 3 protein
UniProt Protein Name:DNA damage-inducible transcript 3 protein
UniProt Synonym Protein Names:C/EBP zeta; C/EBP-homologous protein; CHOP; C/EBP-homologous protein 10; CHOP-10; CCAAT/enhancer-binding protein homologous protein; Growth arrest and DNA damage-inducible protein GADD153
Protein Family:DNA damage-inducible transcript 3 protein
UniProt Gene Name:DDIT3��
UniProt Entry Name:DDIT3_HUMAN

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