Human DCTD Recombinant Protein (RPPB1558)
- SKU:
- RPPB1558
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P32321
- Research Area:
- Enzymes
Description
| Product Name: | Human DCTD Recombinant Protein |
| Product Code: | RPPB1558 |
| Size: | 25µg |
| Species: | Human |
| Target: | DCTD |
| Synonyms: | Deoxycytidylate deaminase, EC 3.5.4.12, dCMP Deaminase, DCTD, MGC111062. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The DCTD solution (0.5mg/ml) contains 20mM Tris-HCl pH-8, 1mM DTT, 1mM EDTA & 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MSEVSCKKRD DYLEWPEYFM AVAFLSAQRS KDPNSQVGAC IVNSENKIVG IGYNGMPNGC SDDVLPWRRT AENKLDTKYP YVCHAELNAI MNKNSTDVKG CSMYVALFPC NECAKLIIQA GIKEVIFMSD KYHDSDEATA ARLLFNMAGV TFRKFIPKCS KIVIDFDSIN SRPSQKLQ |
DCTD is an allosteric enzyme that exists as a homohexamer and is part of the cytidine and deoxycytidylate deaminase protein family. DTCD uses zinc as a cofactor to catalyze the deamination of dCMP to dUMP, thus making the nucleotide substrate (dUMP) that is used by thymidylate synthase.
DCTD Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 198 amino acids (1-178 a.a.) and having a molecular mass of 22.1 kDa. The DCTD is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | DCTD: catalyzes the deamination of dCMP to dUMP, thus providing the nucleotide substrate for thymidylate synthase. Allosteric enzyme whose activity is greatly influenced by the end products of its metabolic pathway, dCTP and dTTP. |
| UniProt Protein Details: | Protein type:Nucleotide Metabolism - pyrimidine; EC 3.5.4.12; Hydrolase Chromosomal Location of Human Ortholog: 4q35.1 Cellular Component: cytosol Molecular Function:zinc ion binding; dCMP deaminase activity Biological Process: pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; nucleotide biosynthetic process; pyrimidine nucleotide metabolic process; pyrimidine nucleoside biosynthetic process |
| NCBI Summary: | The protein encoded by this gene catalyzes the deamination of dCMP to dUMP, the nucleotide substrate for thymidylate synthase. The encoded protein is allosterically activated by dCTP and inhibited by dTTP, and is found as a homohexamer. This protein uses zinc as a cofactor for its activity. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P32321 |
| NCBI GenInfo Identifier: | 23503055 |
| NCBI Gene ID: | 1635 |
| NCBI Accession: | P32321.2 |
| UniProt Secondary Accession: | P32321,Q5M7Z8, Q9BVD8, B2R836, D3DP49, D3DP50, |
| UniProt Related Accession: | P32321 |
| Molecular Weight: | 178 |
| NCBI Full Name: | Deoxycytidylate deaminase |
| NCBI Synonym Full Names: | dCMP deaminase |
| NCBI Official Symbol: | DCTD�� |
| NCBI Protein Information: | deoxycytidylate deaminase |
| UniProt Protein Name: | Deoxycytidylate deaminase |
| UniProt Synonym Protein Names: | dCMP deaminase |
| Protein Family: | Deoxycytidylate deaminase |
| UniProt Gene Name: | DCTD�� |
| UniProt Entry Name: | DCTD_HUMAN |
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