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Human DCPS Recombinant Protein (RPPB1556)

SKU:
RPPB1556
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q96C86
Research Area:
Enzymes
$371
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Description

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Product Name:Human DCPS Recombinant Protein
Product Code:RPPB1556
Size:10µg
Species:Human
Target:DCPS
Synonyms:Scavenger mRNA-decapping enzyme DcpS, DCS-1, Hint-related 7meGMP-directed hydrolase, Histidine triad protein member 5, HINT-5, DCPS, DCS1, HINT5, HSPC015, HSL1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:DCPS protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 20% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MADAAPQLGK RKRELDVEEA HAASTEEKEA GVGNGTCAPV RLPFSGFRLQ KVLRESARDK IIFLHGKVNE ASGDGDGEDA VVILEKTPFQ VEQVAQLLTG SPELQLQFSN DIYSTYHLFP PRQLNDVKTT VVYPATEKHL QKYLRQDLRL IRETGDDYRN ITLPHLESQS LSIQWVYNIL DKKAEADRIV FENPDPSDGF VLIPDLKWNQ QQLDDLYLIA ICHRRGIRSL RDLTPEHLPL LRNILHQGQE AILQRYRMKG DHLRVYLHYL PSYYHLHVHF TALGFEAPGS GVERAHLLAE VIENLECDPR HYQQRTLTFA LRADDPLLKL LQEAQQS

Scavenger mRNA-decapping enzyme (DCPS) is a member of the HIT family. DCPS is required for the complete degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. It was shown that DCPS hydrolyzes the residual m7GpppN cap structure after the complete 3'�5' degradation of the mRNA by the exosome. Furthermore, DCPS releases m7GMP and is incapable of cleaving cap structures attached to a long RNA chain.

DCPS Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 357 amino acids (1-337 a.a.) and having a molecular mass of 40.8kDa.DCPS is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:DCPS: Necessary for the complete degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA fragments shorter than 10 nucleotides that are produced by 3'->5' exosome-mediated mRNA decay. Releases m7GMP. Can also degrade m7GDP to m7GMP. Has no activity towards mRNA molecules longer than 25 nucleotides. Belongs to the HIT family.
UniProt Protein Details:

Protein type:EC 3.6.1.59; Hydrolase; RNA splicing

Chromosomal Location of Human Ortholog: 11q24.2

Cellular Component: cytoplasm; cytosol; mitochondrion; nucleoplasm; nucleus

Molecular Function:exoribonuclease activity; m7G(5')pppN diphosphatase activity; protein binding; RNA 7-methylguanosine cap binding

Biological Process: deadenylation-dependent decapping; mRNA catabolic process, deadenylation-dependent decay; negative regulation of programmed cell death; nuclear mRNA cis splicing, via U2-type spliceosome

Disease: Al-raqad Syndrome

NCBI Summary:This gene encodes a member of the histidine triad family of pyrophosphatases that removes short mRNA fragments containing the 5′ mRNA cap structure, which appear in the 3′ → 5′ mRNA decay pathway, following deadenylation and exosome-mediated turnover. This enzyme hydrolyzes the triphosphate linkage of the cap structure (7-methylguanosine nucleoside triphosphate) to yield 7-methylguanosine monophosphate and nucleoside diphosphate. It protects the cell from the potentially toxic accumulation of these short, capped mRNA fragments, and regulates the activity of other cap-binding proteins, which are inhibited by their accumulation. It also acts as a transcript-specific modulator of pre-mRNA splicing and microRNA turnover. [provided by RefSeq, Apr 2017]
UniProt Code:Q96C86
NCBI GenInfo Identifier:116241325
NCBI Gene ID:28960
NCBI Accession:Q96C86.2
UniProt Secondary Accession:Q96C86,Q8NHL8, Q9Y2S5,
UniProt Related Accession:Q96C86
Molecular Weight:39kDa
NCBI Full Name:m7GpppX diphosphatase
NCBI Synonym Full Names:decapping enzyme, scavenger
NCBI Official Symbol:DCPS��
NCBI Official Synonym Symbols:ARS; DCS1; HSL1; HINT5; HINT-5; HSPC015��
NCBI Protein Information:m7GpppX diphosphatase
UniProt Protein Name:m7GpppX diphosphatase
UniProt Synonym Protein Names:DCS-1; Decapping scavenger enzyme; Hint-related 7meGMP-directed hydrolase; Histidine triad nucleotide-binding protein 5; Histidine triad protein member 5; HINT-5; Scavenger mRNA-decapping enzyme DcpS
Protein Family:m7GpppX diphosphatase
UniProt Gene Name:DCPS��

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