Human DAG1 (Dystrophin Associated Glycoprotein 1) CLIA Kit
The Human DAG1 (Dystrophin-Associated Glycoprotein 1) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of DAG1 levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an ideal tool for researchers studying the role of DAG1 in various health conditions.DAG1 is a vital protein that plays a crucial role in maintaining the integrity of muscle fibers and cell membranes. Mutations in the DAG1 gene have been linked to muscular dystrophies and other neuromuscular disorders, making it a valuable biomarker for diagnosing and monitoring these conditions.
With its high sensitivity and specificity, the Human DAG1 CLIA Kit offers researchers a reliable method for quantifying DAG1 levels in biological samples, allowing for a better understanding of its role in health and disease. This kit is a valuable tool for further research on DAG1 and its implications for muscular dystrophies and other related disorders.
Product Name:
Human DAG1 (Dystrophin Associated Glycoprotein 1) CLIA Kit
SKU:
HUES01283
Target:
Human DAG1 (Dystrophin Associated Glycoprotein 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human DAG1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DAG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DAG1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human DAG1. You can calculate the concentration of Human DAG1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-100
99-115
101-115
Average (%)
93
105
109
1:4
Range (%)
87-99
95-110
84-97
Average (%)
94
100
90
1:8
Range (%)
86-99
87-102
92-104
Average (%)
93
94
99
1:16
Range (%)
95-110
95-112
91-107
Average (%)
101
102
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-105
98
EDTA plasma (n=5)
99-112
105
Cell culture media (n=5)
89-100
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
183.7
640.59
1981.45
185.71
579.16
1843.92
Standard deviation
15.16
65.79
173.18
23.12
62.03
120.96
C V (%)
8.25
10.27
8.74
12.45
10.71
6.56
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human DAG1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human DAG1 in samples. No significant cross-reactivity or interference between Human DAG1 and analogues was observed.
