The Human D2R (DRD2) Dopamine Receptor D2 CLIA Kit is a powerful tool for researchers looking to accurately measure levels of the dopamine receptor D2 in human samples. This kit offers high sensitivity and specificity, ensuring precise and consistent results across serum, plasma, and cell culture supernatants.The dopamine receptor D2 is a key player in the regulation of neurotransmission, impacting various physiological and psychological functions. Dysregulation of this receptor has been implicated in conditions such as schizophrenia, addiction, and Parkinson's disease, making it a valuable target for studying these disorders and developing therapeutic interventions.
Whether you are investigating the role of dopamine signaling in neurological disorders or exploring new treatment options, the Human D2R Dopamine Receptor D2 CLIA Kit provides the reliability and accuracy you need for your research. Trust in this kit to deliver dependable results and advance your understanding of the dopamine receptor D2.
Product Name:
Human D2R/DRD2 (Dopamine Receptor D2) CLIA Kit
SKU:
HUES00698
Target:
Human D2R/DRD2 (Dopamine Receptor D2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human D2R/DRD2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human D2R/DRD2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human D2R/DRD2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human D2R/DRD2. You can calculate the concentration of Human D2R/DRD2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-98
94-112
88-101
Average (%)
90
102
95
1:4
Range (%)
90-104
97-113
94-106
Average (%)
97
105
99
1:8
Range (%)
99-116
90-102
93-105
Average (%)
107
96
99
1:16
Range (%)
94-111
95-108
95-111
Average (%)
102
102
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-111
103
EDTA plasma (n=5)
90-106
96
Cell culture media (n=5)
86-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
220.37
383.7
1849.86
228.19
396.74
1768.54
Standard deviation
20.34
36.53
204.41
24.12
46.78
188.17
C V (%)
9.23
9.52
11.05
10.57
11.79
10.64
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human D2R/DRD2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human D2R/DRD2 in samples. No significant cross-reactivity or interference between Human D2R/DRD2 and analogues was observed.
