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Human Dopamine Receptor D1 (DRD1) ELISA Kit

SKU:
HUDL00871
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P21728
Sensitivity:
0.109ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
DR-D1,D1R,DADR,DRD1A
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Dopamine Receptor D1 (DRD1) ELISA Kit

The Human Dopamine Receptor D1 (DRD1) ELISA Kit is a powerful tool for accurately measuring levels of the DRD1 protein in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.DRD1 is a key receptor in the dopamine system, playing a crucial role in regulating mood, cognition, and motor activity.

Dysregulation of DRD1 has been implicated in various neurological and psychiatric disorders, making it a valuable biomarker for understanding these conditions and developing new therapeutic approaches.With its reliable performance and versatility, the Human Dopamine Receptor D1 ELISA Kit is a valuable asset for researchers studying the dopamine system and its implications in human health and disease.

Product Name:Human Dopamine Receptor D1 (DRD1) ELISA Kit
Product Code:HUDL00871
Size:96 Assays
Target:DRD1
Synonyms:DR-D1, D1R, DADR, DRD1A
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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