The Human DβH (Dopamine-β-Hydroxylase) ELISA Kit is a specialized assay designed for the quantitative measurement of Dopamine-β-Hydroxylase levels in various human biological samples. Dopamine-β-Hydroxylase is a critical enzyme involved in the conversion of dopamine to norepinephrine, playing a crucial role in catecholamine biosynthesis and neurotransmitter metabolism. Accurate and reliable quantification of DβH is essential for understanding its regulatory functions in neurotransmission and other physiological processes.
Our DβH ELISA Kit ensures exceptional sensitivity and specificity, delivering reproducible results with high precision. Manufactance under rigorous quality control protocols, this kit offers robust performance and user-friendly protocols, making it an ideal choice for both research and clinical applications. Rely on Assay Genie's DβH ELISA Kit for precise and dependable quantification of this important enzyme in your studies.
Product Name:
Human DβH (Dopamine-β-Hydroxylase) ELISA Kit
SKU:
AEES00101
Target:
Human DβH (Dopamine-β-Hydroxylase)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 mIU/mL
Detection range:
1.56-100 mIU/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human DβH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DβH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DβH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human DβH. You can calculate the concentration of Human DβH in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
83-96
91-103
95-105
Average (%)
90
96
99
1:4
Range (%)
87-98
90-98
92-104
Average (%)
91
93
96
1:8
Range (%)
96-107
91-105
96-106
Average (%)
100
97
102
1:16
Range (%)
97-107
90-97
85-98
Average (%)
102
94
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-110
103
EDTA plasma (n=5)
95-107
103
Cell culture media (n=5)
97-106
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.11
11.62
45.36
5.0
10.57
41.3
Standard deviation
0.22
0.54
2.37
0.29
0.5
1.78
C V (%)
5.35
4.65
5.22
5.8
4.73
4.31
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human DβH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human DβH in samples. No significant cross-reactivity or interference between Human DβH and analogues was observed.
