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Human Cytokine Array (23 targets) (SARB0034)

SKU:
SARB0034
Product Type:
Protein Array
Analytes:
GCSF
GM-CSF
GRO alpha/beta/gamma
GRO alpha (CXCL1)
IL-1 alpha (IL-1 F1)
IL-2
IL-3
IL-5
IL-6
IL-7
IL-8 (CXCL8)
IL-10
IL-13
IL-15
IFN-gamma
MCP-1 (CCL2)
MCP-2 (CCL8)
MCP-3 (MARC/CCL7)
MIG (CXCL9)
RANTES (CCL5)
TGF beta 1
TNF alpha
TNF beta (TNFSF1B)
Reactivity:
Human
Applications:
Multiplex Array
€669 - €909
Frequently bought together:

Description

system_update_altDatasheet

Human Cytokine Array (23 targets) (SARB0034)

The Human Cytokine Array - 23 Targets (SARB0034) from AssayGenie is a powerful tool for researchers studying cytokine expression levels in human samples. This array allows for the simultaneous detection of 23 different cytokines, providing a comprehensive overview of the immune response in various cell types.With targets including interleukins, chemokines, and growth factors, this array enables researchers to analyze the complex interplay of cytokines in immune regulation, inflammation, and disease.

The array is easy to use, requiring only a small amount of sample for analysis, and produces reliable and reproducible results.Whether studying cancer, autoimmune disorders, or infectious diseases, the Human Cytokine Array - 23 Targets offers valuable insights into the cytokine profiles associated with different conditions. With its high sensitivity and specificity, this array is a valuable tool for any researcher seeking to unravel the intricate workings of the immune system.

Product Sku:SARB0034
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:23
Gene Symbols:CCL2, CCL5, CCL7, CCL8, CSF2, CSF3, CXCL1, CXCL8, CXCL9, IFNG|IL10, IL13, IL15, IL1A, IL2, IL3, IL5, IL6, IL7, LTA, TGFB1, TNF
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
GCSF
  • Gene Symbol: CSF3 C17orf33 GCSF
  • Gene ID: 1440
  • Accession #: P09919
GM-CSF
  • Gene Symbol: CSF2 GMCSF
  • Gene ID: 1437
  • Accession #: P04141
GRO alpha/beta/gamma
  • Gene Symbol: N/A
  • Gene ID: N/A
  • Accession #: P09341/P19875/P19876
GRO alpha (CXCL1)
  • Gene Symbol: CXCL1 GRO GRO1 GROA MGSA SCYB1
  • Gene ID: 2919
  • Accession #: P09341
IL-1 alpha (IL-1 F1)
  • Gene Symbol: IL1A IL1F1
  • Gene ID: 3552
  • Accession #: P01583
IL-2
  • Gene Symbol: IL2
  • Gene ID: 3558
  • Accession #: P60568
IL-3
  • Gene Symbol: IL3
  • Gene ID: 3562
  • Accession #: P08700
IL-5
  • Gene Symbol: IL5
  • Gene ID: 3567
  • Accession #: P05113
IL-6
  • Gene Symbol: IL6 IFNB2
  • Gene ID: 3569
  • Accession #: P05231
IL-7
  • Gene Symbol: IL7
  • Gene ID: 3574
  • Accession #: P13232
IL-8 (CXCL8)
  • Gene Symbol: CXCL8 IL8
  • Gene ID: 3576
  • Accession #: P10145
IL-10
  • Gene Symbol: IL10
  • Gene ID: 3586
  • Accession #: P22301
IL-13
  • Gene Symbol: IL13 NC30
  • Gene ID: 3596
  • Accession #: P35225
IL-15
  • Gene Symbol: IL15
  • Gene ID: 3600
  • Accession #: P40933
IFN-gamma
  • Gene Symbol: IFNG
  • Gene ID: 3458
  • Accession #: P01579
MCP-1 (CCL2)
  • Gene Symbol: CCL2 MCP1 SCYA2
  • Gene ID: 6347
  • Accession #: P13500
MCP-2 (CCL8)
  • Gene Symbol: CCL8 MCP2 SCYA10 SCYA8
  • Gene ID: 6355
  • Accession #: P80075
MCP-3 (MARC/CCL7)
  • Gene Symbol: CCL7 MCP3 SCYA6 SCYA7
  • Gene ID: 6354
  • Accession #: P80098
MIG (CXCL9)
  • Gene Symbol: CXCL9 CMK MIG SCYB9
  • Gene ID: 4283
  • Accession #: Q07325
RANTES (CCL5)
  • Gene Symbol: CCL5 D17S136E SCYA5
  • Gene ID: 6352
  • Accession #: P13501
TGF beta 1
  • Gene Symbol: TGFB1 TGFB
  • Gene ID: 7040
  • Accession #: P01137
TNF alpha
  • Gene Symbol: TNF TNFA TNFSF2
  • Gene ID: 7124
  • Accession #: P01375
TNF beta (TNFSF1B)
  • Gene Symbol: LTA TNFB TNFSF1
  • Gene ID: 4049
  • Accession #: P01374
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Human Cytokine Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis

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