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Human Cytochrome P450 27A1 (CYP27A1) ELISA Kit

SKU:
HUDL00765
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q02318
Sensitivity:
0.061ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CP27,CTX,CYP27,Sterol 27-Hydroxylase,Cerebrotendinous Xanthomatosis,Vitamin D(3) 25-hydroxylase,5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 27-hydroxylase
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

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Human Cytochrome P450 27A1 (CYP27A1) ELISA Kit

The Human Cytochrome P450 27A1 (CYP27A1) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of CYP27A1 levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it a valuable tool for a wide range of research applications.Cytochrome P450 27A1 is an important enzyme involved in the metabolism of cholesterol and bile acids, playing a crucial role in maintaining lipid homeostasis.

Dysregulation of CYP27A1 has been linked to various diseases, including cardiovascular diseases, neurological disorders, and metabolic disorders. Therefore, the Human CYP27A1 ELISA Kit is an essential tool for studying these conditions and exploring potential therapeutic interventions.Overall, the Human CYP27A1 ELISA Kit offers researchers a reliable and efficient method for quantifying CYP27A1 levels, advancing our understanding of its role in health and disease.

Product Name:Human Cytochrome P450 27A1 (CYP27A1) ELISA Kit
Product Code:HUDL00765
Size:96 Assays
Target:CYP27A1
Synonyms:CP27, CTX, CYP27, Sterol 27-Hydroxylase,Cerebrotendinous Xanthomatosis, Vitamin D(3) 25-hydroxylase, 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 27-hydroxylase
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10191
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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