null

Human Cytochrome P450 26A1 (CYP26A1) ELISA Kit

SKU:
HUDL00764
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O43174
Sensitivity:
0.055ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CP26,CYP26,P450RAI,P450RAI1,hP450RAI,Cytochrome P450 retinoic acid-inactivating 1,Retinoic acid 4-hydroxylase,Retinoic acid-metabolizing cytochrome
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Cytochrome P450 26A1 (CYP26A1) ELISA Kit

The Human Cytochrome P450 26A1 (CYP26A1) ELISA Kit is specifically designed for the accurate quantification of CYP26A1 levels in human biological samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.Cytochrome P450 26A1 is an important enzyme involved in the metabolism of retinoic acid, a key regulator of various biological processes such as cell differentiation, growth, and development.

Dysregulation of CYP26A1 has been associated with various diseases including cancer, autoimmune disorders, and developmental disorders, highlighting its significance as a potential biomarker for disease research and drug development.With its superior performance and ease of use, the Human Cytochrome P450 26A1 ELISA Kit is a valuable tool for researchers studying the role of CYP26A1 in health and disease, and for identifying potential therapeutic targets for various medical conditions.

Product Name:Human Cytochrome P450 26A1 (CYP26A1) ELISA Kit
Product Code:HUDL00764
Size:96 Assays
Target:CYP26A1
Synonyms:CP26, CYP26, P450RAI, P450RAI1, hP450RAI, Cytochrome P450 retinoic acid-inactivating 1, Retinoic acid 4-hydroxylase, Retinoic acid-metabolizing cytochrome
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10191
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products