null

Human Cytochrome P450 11B1 (CYP11B1) ELISA Kit

SKU:
HUDL00759
Product Type:
ELISA Kit
Sensitivity:
0.074ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CPN1,CYP11B,FHI,CYPXIB1,S11BH,11-Beta-Hydroxylase,Cytochrome P450 Family 11 Subfamily B Polypeptide 1,Steroid 11-beta-hydroxylase,Cytochrome P-450c11
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Human Cytochrome P450 11B1 (CYP11B1) ELISA Kit

The Human Cytochrome P450 11B1 (CYP11B1) ELISA Kit is specifically designed for the precise measurement of CYP11B1 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.Cytochrome P450 11B1 (CYP11B1) is a crucial enzyme involved in the biosynthesis of steroid hormones, playing a significant role in the regulation of various physiological processes.

Dysregulation of CYP11B1 has been linked to disorders such as adrenal insufficiency and hypertension, making it a key target for understanding these conditions and developing potential interventions.Overall, the Human Cytochrome P450 11B1 (CYP11B1) ELISA Kit provides researchers with a valuable tool for studying the role of this enzyme in human health and disease, offering reliable and reproducible data for further investigation and therapeutic development.

Product Name:Human Cytochrome P450 11B1 (CYP11B1) ELISA Kit
Product Code:HUDL00759
Size:96 Assays
Target:CYP11B1
Synonyms:CPN1, CYP11B, FHI, CYPXIB1, S11BH, 11-Beta-Hydroxylase, Cytochrome P450 Family 11 Subfamily B Polypeptide 1, Steroid 11-beta-hydroxylase, Cytochrome P-450c11
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10191
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products