The Cys-C ELISA Kit is designed for the quantitative detection of Cystatin C (Cys-C) levels in various human biological samples. Cys-C is a major extracellular inhibitor of cysteine proteases and plays a significant role in protein catabolism, cell signaling, and inflammatory response. Its elevated levels are often associated with renal diseases and certain types of cancers.
Accurate measurement of Cys-C is crucial for understanding its role in disease mechanisms and for developing targeted therapeutic strategies. Assay Genie's Cys-C ELISA Kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under stringent quality control standards, this kit delivers robust performance and is easy to use, making it an excellent choice for both research and clinical applications. Trust Assay Genie's Cys-C ELISA Kit for accurate and dependable quantification of this critical biomarker in your studies.
Product Name:
Human Cys-C (Cystatin C) ELISA Kit
SKU:
AEES00039
Target:
Human Cys-C (Cystatin C)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Cys-C. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Cys-C and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Cys-C, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Cys-C. You can calculate the concentration of Human Cys-C in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
83-98
87-102
89-102
Average (%)
90
93
95
1:4
Range (%)
99-115
82-94
95-109
Average (%)
105
89
103
1:8
Range (%)
99-117
85-98
94-108
Average (%)
107
91
101
1:16
Range (%)
95-111
81-95
96-114
Average (%)
102
87
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-105
98
EDTA plasma (n=5)
94-110
100
Cell culture media (n=5)
92-101
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.3
6.8
15.6
2.3
7.5
15.6
Standard deviation
0.09
0.12
0.23
0.1
0.16
0.24
C V (%)
4.55
4.22
5.28
6.13
5.73
4.29
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human Cys-C concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Cys-C in samples. No significant cross-reactivity or interference between Human Cys-C and analogues was observed.
