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Human CYR61 Recombinant Protein (RPPB0153)

SKU:
RPPB0153
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
O00622
Research Area:
Growth Factors & Cytokines
€309
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Description

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Product Name:Human CYR61 Recombinant Protein
Product Code:RPPB0153
Size:20µg
Species:Human
Target:CYR61
Synonyms:CYR61, Protein CYR61, Cysteine-rich angiogenic inducer 61, IGF-binding protein 10, IGFBP-10, IBP-10, Protein GIG1, CCN family member 1, CCN1, GIG1, IGFBP10.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:Lyophilized from a 0.2m filtered concentrated solution in PBS, pH 7.4.
Solubility:It is recommended to reconstitute the lyophilized CYR61 in sterile 18M-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized CYR61 Human although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CYR61 should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:TCPAACHCPL EAPKCAPGVG LVRDGCGCCK VCAKQLNEDC SKTQPCDHTK GLECNFGASS TALKGICRAQ SEGRPCEYNS RIYQNGESFQ PNCKHQCTCI DGAVGCIPLC PQELSLPNLG CPNPRLVKVT GQCCEEWVCD EDSIKDPMED QDGLLGKELG FDASEVELTR NNELIAVGKG SSLKRLPVFG MEPRILYNPL QGQKCIVQTT SWSQCSKTCG TGISTRVTND NPECRLVKET RICEVRPCGQ PVYSSLKKGK KCSKTKKSPE PVRFTYAGCL SVKKYRPKYC GSCVDGRCCTPQLTRTVKMR FRCEDGETFS KNVMMIQSCK CNYNCPHANE AAFPFYRLFN DIHKFRD
Biological Activity:The ED50 was determined by the proliferation of mouse 3T3 cells is < 2.0 ug/ml, corresponding to a specific activity of > 500 units/mg.

CYR61 is a growth factor-inducible, immediate-early gene that has multifaceted activities in various cancers. CYR61 is a secreted, cysteine-rich, binding protein which is encoded by a growth factor-inducible immediate-early gene. Acting as an extracellular, matrix-associated signaling molecule, CYR61 promotes the adhesion of endothelial cells through interaction with integrin and enhances growth factor-induced DNA synthesis in the same cell type.

CYR61 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 357 amino acids and having a molecular mass of 39.5kDa.The CYR61 is purified by proprietary chromatographic techniques.

UniProt Protein Function:CYR61: Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up- regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3. Belongs to the CCN family.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1p22.3

Cellular Component: extracellular matrix; proteinaceous extracellular matrix

Molecular Function:heparin binding; integrin binding; insulin-like growth factor binding; extracellular matrix binding

Biological Process: anatomical structure morphogenesis; extracellular matrix organization and biogenesis; positive regulation of caspase activity; chemotaxis; intussusceptive angiogenesis; osteoblast differentiation; cell proliferation; positive regulation of osteoblast differentiation; cell-cell signaling; positive regulation of protein kinase activity; positive regulation of osteoblast proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; regulation of cell growth; cell adhesion; positive regulation of BMP signaling pathway; positive regulation of cell migration; negative regulation of apoptosis

NCBI Summary:The secreted protein encoded by this gene is growth factor-inducible and promotes the adhesion of endothelial cells. The encoded protein interacts with several integrins and with heparan sulfate proteoglycan. This protein also plays a role in cell proliferation, differentiation, angiogenesis, apoptosis, and extracellular matrix formation. [provided by RefSeq, Sep 2011]
UniProt Code:O00622
NCBI GenInfo Identifier:3023601
NCBI Gene ID:3491
NCBI Accession:O00622.1
UniProt Secondary Accession:O00622,O14934, O43775, Q9BZL7,
UniProt Related Accession:O00622
Molecular Weight:
NCBI Full Name:Protein CYR61
NCBI Synonym Full Names:cysteine-rich, angiogenic inducer, 61
NCBI Official Symbol:CYR61��
NCBI Official Synonym Symbols:CCN1; GIG1; IGFBP10��
NCBI Protein Information:protein CYR61; IBP-10; IGFBP-10; CCN family member 1; IGF-binding protein 10; cysteine-rich, anigogenic inducer, 61; cysteine-rich heparin-binding protein 61; insulin-like growth factor-binding protein 10
UniProt Protein Name:Protein CYR61
UniProt Synonym Protein Names:CCN family member 1; Cysteine-rich angiogenic inducer 61; Insulin-like growth factor-binding protein 10; IBP-10; IGF-binding protein 10; IGFBP-10; Protein GIG1
Protein Family:Protein
UniProt Gene Name:CYR61��
UniProt Entry Name:CYR61_HUMAN

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