Human CYP26A1 (Cytochrome P450, family 26, subfamily A, polypeptide 1) CLIA Kit
The Human CYP26A1 (Cytochrome P450 Family 26 Subfamily A Polypeptide 1) CLIA Kit is a reliable and precise tool for the quantitative measurement of CYP26A1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research purposes.CYP26A1 is a key enzyme involved in the metabolism of retinoic acid, playing a critical role in regulating gene expression and development processes. Dysregulation of CYP26A1 has been implicated in various diseases, including cancer, developmental disorders, and inflammatory conditions.
Therefore, accurate measurement of CYP26A1 levels is essential for studying its role in disease pathogenesis and for identifying potential therapeutic targets.With its advanced technology and user-friendly format, the Human CYP26A1 CLIA Kit from AssayGenie is the perfect choice for researchers looking to explore the functions of CYP26A1 and its impact on human health. Get reliable and reproducible results with this innovative kit for your research needs.
Product Name:
Human CYP26A1 (Cytochrome P450, family 26, subfamily A, polypeptide 1) CLIA Kit
SKU:
HUES00296
Target:
Human CYP26A1 (Cytochrome P450, family 26, subfamily A, polypeptide 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CYP26A1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CYP26A1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CYP26A1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CYP26A1. You can calculate the concentration of Human CYP26A1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-106
95-111
101-114
Average (%)
99
101
108
1:4
Range (%)
100-114
101-113
85-96
Average (%)
108
107
91
1:8
Range (%)
90-105
89-103
89-103
Average (%)
97
95
95
1:16
Range (%)
91-104
91-103
87-101
Average (%)
97
96
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-106
98
EDTA plasma (n=5)
86-96
91
Cell culture media (n=5)
94-108
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
107.07
266.68
764.75
115.24
255.66
802.44
Standard deviation
11.86
24.53
68.14
12.5
27.87
70.29
C V (%)
11.08
9.2
8.91
10.85
10.9
8.76
Sample type &Sample volume:
Tissue homogenates; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CYP26A1 concentrations in Tissue homogenates.
Specificity:
This kit recognizes Human CYP26A1 in samples. No significant cross-reactivity or interference between Human CYP26A1 and analogues was observed.
