Human CYP1A2 (Cytochrome P450, family 1, subfamily A, polypeptide 2) CLIA Kit
The Human CYP1A2 (Cytochrome P450 Family 1 Subfamily A Polypeptide 2) CLIA Kit is a precise assay designed for the accurate measurement of CYP1A2 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring dependable and consistent results for a variety of research purposes.CYP1A2 is a key enzyme involved in drug metabolism and detoxification, playing a crucial role in the biotransformation of numerous medications and environmental toxins.
Dysregulation of CYP1A2 has been linked to various health conditions including drug interactions, cancer, and metabolic disorders, making it an important biomarker for studying these diseases and developing targeted therapies.Overall, the Human CYP1A2 CLIA Kit provides researchers with a valuable tool for investigating the role of CYP1A2 in human health and disease, offering a reliable way to analyze CYP1A2 expression levels and activity in biological samples.
Product Name:
Human CYP1A2 (Cytochrome P450, family 1, subfamily A, polypeptide 2) CLIA Kit
SKU:
HUES00261
Target:
Human CYP1A2 (Cytochrome P450, family 1, subfamily A, polypeptide 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CYP1A2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CYP1A2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CYP1A2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CYP1A2. You can calculate the concentration of Human CYP1A2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-104
102-116
85-99
Average (%)
98
109
91
1:4
Range (%)
85-97
93-107
95-108
Average (%)
91
99
101
1:8
Range (%)
95-113
101-117
93-106
Average (%)
103
108
98
1:16
Range (%)
99-114
84-99
100-120
Average (%)
106
90
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
96
EDTA plasma (n=5)
84-95
90
Cell culture media (n=5)
88-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
101.25
297.42
900.22
95.56
319.21
920.75
Standard deviation
10.89
24.51
61.3
10.25
29.62
85.81
C V (%)
10.76
8.24
6.81
10.73
9.28
9.32
Sample type &Sample volume:
Tissue homogenates; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CYP1A2 concentrations in Tissue homogenates.
Specificity:
This kit recognizes Human CYP1A2 in samples. No significant cross-reactivity or interference between Human CYP1A2 and analogues was observed.
