Human CYP1A1 (Cytochrome P450, family 1, subfamily A, polypeptide 1) ELISA Kit
The Human CYP1A1 (Cytochrome P450, family 1, subfamily A, polypeptide 1) ELISA Kit is specifically designed for the quantitative detection of CYP1A1 levels in human biological samples. CYP1A1, a member of the cytochrome P450 enzyme family, is crucial for the metabolism of various endogenous and exogenous compounds, including drugs, toxins, and environmental chemicals. This enzyme plays a vital role in xenobiotic metabolism, hormone synthesis, and detoxification processes in the body. Accurate measurement of CYP1A1 levels is essential for understanding its involvement in drug metabolism, environmental toxin processing, and the body's response to oxidative stress.
This ELISA kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results. With robust performance and ease of use, this kit is ideal for both research and clinical applications. Trust the Assay Genie's CYP1A1 ELISA Kit for reliable quantification of this important enzyme in your studies.
Product Name:
Human CYP1A1 (Cytochrome P450, family 1, subfamily A, polypeptide 1) ELISA Kit
SKU:
AEES00072
Target:
Human CYP1A1 (Cytochrome P450, family 1, subfamily A, polypeptide 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CYP1A1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CYP1A1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CYP1A1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CYP1A1. You can calculate the concentration of Human CYP1A1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-99
87-98
86-101
Average (%)
94
95
94
1:4
Range (%)
92-98
96-102
88-101
Average (%)
95
99
93
1:8
Range (%)
87-102
96-108
90-101
Average (%)
95
103
93
1:16
Range (%)
89-97
92-104
89-100
Average (%)
94
96
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
96
EDTA plasma (n=5)
91-102
97
Cell culture media (n=5)
83-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.84
2.26
9.41
0.82
2.25
8.36
Standard deviation
0.05
0.11
0.44
0.05
0.11
0.35
C V (%)
5.95
4.87
4.68
6.1
4.89
4.19
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CYP1A1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CYP1A1 in samples. No significant cross-reactivity or interference between Human CYP1A1 and analogues was observed.
