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Human Cyclin Dependent Kinase 4 (CDK4) ELISA Kit

SKU:
HUDL00515
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P11802
Sensitivity:
0.121ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
CMM3,PSK-J3,Cell division protein kinase 4
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Human Cyclin Dependent Kinase 4 (CDK4) ELISA Kit

The Human Cyclin-Dependent Kinase 4 (CDK4) ELISA Kit is specifically designed for the accurate measurement of CDK4 levels in human serum, plasma, and cell culture supernatants. This kit is characterized by its high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.CDK4 is a key enzyme involved in cell cycle regulation, playing a crucial role in cell division and proliferation.

Dysregulation of CDK4 activity has been implicated in various diseases, including cancer, making it an important biomarker for studying disease mechanisms and potential therapeutic interventions.With its advanced technology and ease of use, the Human CDK4 ELISA Kit is an essential tool for researchers and clinicians alike in investigating the role of CDK4 in health and disease.

Product Name:Human Cyclin Dependent Kinase 4 (CDK4) ELISA Kit
Product Code:HUDL00515
Size:96 Assays
Target:CDK4
Synonyms:CMM3, PSK-J3, Cell division protein kinase 4
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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