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Human Cyclin Dependent Kinase 2 (CDK2) ELISA Kit

SKU:
HUDL00514
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P24941
Sensitivity:
0.113ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
CDKN2,p33(CDK2),Cell division protein kinase 2,p33 protein kinase
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Cyclin Dependent Kinase 2 (CDK2) ELISA Kit

The Human Cyclin Dependent Kinase 2 (CDK2) ELISA Kit is a powerful tool for accurate quantification of CDK2 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it ideal for various research applications in the field of cell cycle regulation and cancer research.CDK2 is a key enzyme involved in the regulation of cell cycle progression and proliferation. Dysregulation of CDK2 activity has been implicated in various diseases, including cancer and neurodegenerative disorders.

By targeting CDK2, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially develop novel therapeutic strategies.Overall, the Human CDK2 ELISA Kit offers researchers a powerful tool to accurately measure CDK2 levels, paving the way for groundbreaking discoveries in cell biology and disease research.

Product Name:Human Cyclin Dependent Kinase 2 (CDK2) ELISA Kit
Product Code:HUDL00514
Size:96 Assays
Target:CDK2
Synonyms:CDKN2, p33(CDK2), Cell division protein kinase 2, p33 protein kinase
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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