Human CYB5R2 Recombinant Protein (RPPB1530)
- SKU:
- RPPB1530
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q6BCY4
- Research Area:
- Enzymes
Description
| Product Name: | Human CYB5R2 Recombinant Protein |
| Product Code: | RPPB1530 |
| Size: | 20µg |
| Species: | Human |
| Target: | CYB5R2 |
| Synonyms: | CYB5R2, Cytochrome B5 Reductase 2, EC 1.6.2.2, B5R.2, Cytochrome B5 Reductase B5R.2, NADH-Cytochrome B5 Reductase 2, b5R.2. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | CYB5R2 protein solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMNSRRRE PITLQDPEAK YPLPLIEKEK ISHNTRRFRF GLPSPDHVLG LPVGNYVQLL AKIDNELVVR AYTPVSSDDD RGFVDLIIKI YFKNVHPQYP EGGKMTQYLE NMKIGETIFF RGPRGRLFYH GPGNLGIRPD QTSEPKKTLA DHLGMIAGGT GITPMLQLIR HITKDPSDRT RMSLIFANQT EEDILVRKEL EEIARTHPDQ FNLWYTLDRP PIGWKYSSGF VTADMIKEHL PPPAKSTLIL VCGPPPLIQT AAHPNLEKLG YTQDMIFTY |
Cytochrome b5 reductase 2 (CYB5R2) is involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. CYB5R2 is responsible for NADH-dependent lucigenin chemiluminescence in spermatozoa by reducing both lucigenin and 2-[4-iodophenyl]-3-[4-nitrophenyl]-5-[2,4-disulfophenyl]-2H tetrazolium monosodium salt (WST-1).
CYB5R2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 299 amino acids (1-276 a.a.) and having a molecular mass of 33.8kDa. CYB5R2 is fused to a 23 amino acid His-tag at N-terminus.
| UniProt Protein Function: | CYB5R2: NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. Responsible for NADH-dependent lucigenin chemiluminescence in spermatozoa by reducing both lucigenin and 2-[4-iodophenyl]-3-[4-nitrophenyl]-5-[2,4- disulfophenyl]-2H tetrazolium monosodium salt (WST-1). Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Oxidoreductase; EC 1.6.2.2 Chromosomal Location of Human Ortholog: 11p15.4 Cellular Component: endoplasmic reticulum membrane; membrane; nucleus Molecular Function:cytochrome-b5 reductase activity; protein binding Biological Process: bicarbonate transport |
| NCBI Summary: | The protein encoded by this gene belongs to the flavoprotein pyridine nucleotide cytochrome reductase family of proteins. Cytochrome b-type NAD(P)H oxidoreductases are implicated in many processes including cholesterol biosynthesis, fatty acid desaturation and elongation, and respiratory burst in neutrophils and macrophages. Cytochrome b5 reductases have soluble and membrane-bound forms that are the product of alternative splicing. In animal cells, the membrane-bound form binds to the endoplasmic reticulum, where it is a member of a fatty acid desaturation complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014] |
| UniProt Code: | Q6BCY4 |
| NCBI GenInfo Identifier: | 74709211 |
| NCBI Gene ID: | 51700 |
| NCBI Accession: | Q6BCY4.1 |
| UniProt Secondary Accession: | Q6BCY4,Q9BVA3, Q9UF68, Q9UHJ0, |
| UniProt Related Accession: | Q6BCY4 |
| Molecular Weight: | 27,043 Da |
| NCBI Full Name: | NADH-cytochrome b5 reductase 2 |
| NCBI Synonym Full Names: | cytochrome b5 reductase 2 |
| NCBI Official Symbol: | CYB5R2�� |
| NCBI Official Synonym Symbols: | B5R.2�� |
| NCBI Protein Information: | NADH-cytochrome b5 reductase 2 |
| UniProt Protein Name: | NADH-cytochrome b5 reductase 2 |
| Protein Family: | NADH-cytochrome b5 reductase |
| UniProt Gene Name: | CYB5R2�� |
| UniProt Entry Name: | NB5R2_HUMAN |
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