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Human CYB5R2 Recombinant Protein (RPPB1530)

SKU:
RPPB1530
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q6BCY4
Research Area:
Enzymes
€309
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Description

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Product Name:Human CYB5R2 Recombinant Protein
Product Code:RPPB1530
Size:20µg
Species:Human
Target:CYB5R2
Synonyms:CYB5R2, Cytochrome B5 Reductase 2, EC 1.6.2.2, B5R.2, Cytochrome B5 Reductase B5R.2, NADH-Cytochrome B5 Reductase 2, b5R.2.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:CYB5R2 protein solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMNSRRRE PITLQDPEAK YPLPLIEKEK ISHNTRRFRF GLPSPDHVLG LPVGNYVQLL AKIDNELVVR AYTPVSSDDD RGFVDLIIKI YFKNVHPQYP EGGKMTQYLE NMKIGETIFF RGPRGRLFYH GPGNLGIRPD QTSEPKKTLA DHLGMIAGGT GITPMLQLIR HITKDPSDRT RMSLIFANQT EEDILVRKEL EEIARTHPDQ FNLWYTLDRP PIGWKYSSGF VTADMIKEHL PPPAKSTLIL VCGPPPLIQT AAHPNLEKLG YTQDMIFTY

Cytochrome b5 reductase 2 (CYB5R2) is involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. CYB5R2 is responsible for NADH-dependent lucigenin chemiluminescence in spermatozoa by reducing both lucigenin and 2-[4-iodophenyl]-3-[4-nitrophenyl]-5-[2,4-disulfophenyl]-2H tetrazolium monosodium salt (WST-1).

CYB5R2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 299 amino acids (1-276 a.a.) and having a molecular mass of 33.8kDa. CYB5R2 is fused to a 23 amino acid His-tag at N-terminus.

UniProt Protein Function:CYB5R2: NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. Responsible for NADH-dependent lucigenin chemiluminescence in spermatozoa by reducing both lucigenin and 2-[4-iodophenyl]-3-[4-nitrophenyl]-5-[2,4- disulfophenyl]-2H tetrazolium monosodium salt (WST-1). Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Oxidoreductase; EC 1.6.2.2

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: endoplasmic reticulum membrane; membrane; nucleus

Molecular Function:cytochrome-b5 reductase activity; protein binding

Biological Process: bicarbonate transport

NCBI Summary:The protein encoded by this gene belongs to the flavoprotein pyridine nucleotide cytochrome reductase family of proteins. Cytochrome b-type NAD(P)H oxidoreductases are implicated in many processes including cholesterol biosynthesis, fatty acid desaturation and elongation, and respiratory burst in neutrophils and macrophages. Cytochrome b5 reductases have soluble and membrane-bound forms that are the product of alternative splicing. In animal cells, the membrane-bound form binds to the endoplasmic reticulum, where it is a member of a fatty acid desaturation complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]
UniProt Code:Q6BCY4
NCBI GenInfo Identifier:74709211
NCBI Gene ID:51700
NCBI Accession:Q6BCY4.1
UniProt Secondary Accession:Q6BCY4,Q9BVA3, Q9UF68, Q9UHJ0,
UniProt Related Accession:Q6BCY4
Molecular Weight:27,043 Da
NCBI Full Name:NADH-cytochrome b5 reductase 2
NCBI Synonym Full Names:cytochrome b5 reductase 2
NCBI Official Symbol:CYB5R2��
NCBI Official Synonym Symbols:B5R.2��
NCBI Protein Information:NADH-cytochrome b5 reductase 2
UniProt Protein Name:NADH-cytochrome b5 reductase 2
Protein Family:NADH-cytochrome b5 reductase
UniProt Gene Name:CYB5R2��
UniProt Entry Name:NB5R2_HUMAN

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