The Human CXCR3 (Chemokine CXC Receptor 3) ELISA Kit is specifically designed for the precise measurement of CXCR3 levels in human samples including serum, plasma, and cell culture supernatants. This kit is known for its superior sensitivity and specificity, ensuring dependable and consistent results for various research applications.CXCR3 is a key chemokine receptor known to play a critical role in inflammatory responses, immune cell migration, and modulation of immune cell functions. Dysregulation of CXCR3 has been implicated in various diseases including autoimmune disorders, inflammatory conditions, and cancer, highlighting its importance as a potential biomarker for disease research and therapeutic development.
The Human CXCR3 ELISA Kit from AssayGenie is a reliable tool for researchers seeking to study the role of CXCR3 in health and disease, providing accurate and quantitative data to further our understanding of this important chemokine receptor. Order yours today to advance your research in the field of immunology and inflammation.
Product Name:
Human CXCR3 (Chemokine CXC Receptor 3) ELISA Kit
SKU:
HUES01985
Target:
Human CXCR3 (Chemokine CXC Receptor 3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CXCR3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CXCR3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CXCR3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CXCR3. You can calculate the concentration of Human CXCR3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-111
92-102
91-101
Average (%)
102
97
96
1:4
Range (%)
88-101
81-92
88-101
Average (%)
95
88
93
1:8
Range (%)
91-104
83-97
83-97
Average (%)
98
89
88
1:16
Range (%)
86-99
82-94
86-97
Average (%)
93
88
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
92
EDTA plasma (n=5)
94-105
100
Cell culture media (n=5)
96-106
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.49
0.98
4.18
0.5
1.06
3.78
Standard deviation
0.03
0.05
0.15
0.03
0.05
0.16
C V (%)
6.12
5.1
3.59
6.0
4.72
4.23
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CXCR3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CXCR3 in samples. No significant cross-reactivity or interference between Human CXCR3 and analogues was observed.
