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Human CXCL7 / NAP-2 ELISA Kit

SKU:
HUFI00007
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P02775
Sensitivity:
9.375pg/ml
Range:
15.625-1000pg/ml
ELISA Type:
Sandwich
Synonyms:
CXCL7, CTAP III, NAP-2, PPBP, connective tissue-activating peptide III, CTAP3CTAP-III, CTAPIII, CXC chemokine ligand 7, C-X-C motif chemokine 7, CXCL7b-TG1, LA-PF4, LDGFTC1, Leukocyte-derived growth factor, low-affinity platelet factor IV, Macrophage
Reactivity:
Human
Research Area:
Cell Biology
Signaling Molecule:
Chemokine
€599
Frequently bought together:

Description

Human CXCL7/NAP-2 ELISA Kit

The Human CXCL7/NAP-2 ELISA Kit is specially designed for the precise measurement of CXCL7/NAP-2 levels in human serum, plasma, and cell culture supernatants. This kit offers unmatched sensitivity and specificity, ensuring accurate and consistent results for various research purposes.CXCL7/NAP-2, also known as neutrophil-activating protein-2, is a key chemokine involved in the regulation of inflammation and immune response. It plays a critical role in recruiting and activating neutrophils, contributing to the body's defense against infections and injury.

Dysregulation of CXCL7/NAP-2 expression has been linked to various inflammatory and autoimmune diseases, making it a valuable biomarker for understanding disease pathogenesis and developing therapeutic interventions.With its advanced features and proven performance, the Human CXCL7/NAP-2 ELISA Kit is an essential tool for researchers studying inflammatory processes, immune responses, and related diseases. Get reliable and consistent results with this innovative kit from Assay Genie.

Product Name:Human CXCL7 / NAP-2 ELISA Kit
Product Code:HUFI00007
Size:96 Assays
Alias:CXCL7, CTAP III, NAP-2, PPBP, connective tissue-activating peptide III, CTAP3CTAP-III, CTAPIII, CXC chemokine ligand 7, C-X-C motif chemokine 7, CXCL7b-TG1, LA-PF4
Detection method:Sandwich ELISA, Double Antibody
Application:This immunoassay kit allows for the in vitro quantitative determination of Human CXCL7 concentrations in serum plasma and other biological fluids.
Sensitivity:9.375pg/ml
Range:15.625-1000pg/ml
Storage:4°C for 6 months
Note:For Research Use Only
Recovery:Matrices listed below were spiked with certain level of Human CXCL7 and the recovery rates were calculated by comparing the measured value to the expected amount of Human CXCL7 in samples.
MatrixRecovery range(%)Average(%)
serum(n=5)86-10395
EDTA plasma(n=5)85-10594
UFH plasma(n=5)85-10190
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human CXCL7 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample1:21:41:8
serum(n=5)85-95%89-105%86-102%
EDTA plasma(n=5)86-100%86-95%82-99%
UFH plasma(n=5)81-97%80-97%81-96%
CV(%):Intra-Assay: CV<8%
Inter-Assay: CV<10%
ComponentQuantityStorage
ELISA Microplate (Dismountable)8×12 strips4°C for 6 months
Lyophilized Standard24°C/-20°C
Sample/Standard Dilution Buffer20ml4°C
Biotin-labeled Antibody(Concentrated)120ul4°C (Protect from light)
Antibody Dilution Buffer10ml4°C
HRP-Streptavidin Conjugate(SABC)120ul4°C (Protect from light)
SABC Dilution Buffer10ml4°C
TMB Substrate10ml4°C (Protect from light)
Stop Solution10ml4°C
Wash Buffer(25X)30ml4°C
Plate Sealer5-

Other materials and equipment required:

  • Microplate reader with 450 nm wavelength filter
  • Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
  • Incubator
  • Deionized or distilled water
  • Absorbent paper
  • Buffer resevoir
UniprotP02775
UniProt Protein Function:PPBP: LA-PF4 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. NAP-2 is a ligand for CXCR1 and CXCR2, and NAP-2, NAP-2(73), NAP-2(74), NAP-2(1-66), and most potent NAP-2(1-63) are chemoattractants and activators for neutrophils. TC-1 and TC-2 are antibacterial proteins, in vitro released from activated platelet alpha-granules. CTAP-III(1-81) is more potent than CTAP-III desensitize chemokine-induced neutrophil activation. Belongs to the intercrine alpha (chemokine CxC) family.
UniProt Protein Details:Protein type:Secreted, signal peptide; Secreted; Chemokine

Chromosomal Location of Human Ortholog: 4q12-q13

Cellular Component: extracellular space; extracellular region

Molecular Function:growth factor activity; chemokine activity; CXCR chemokine receptor binding; glucose transmembrane transporter activity

Biological Process: platelet activation; response to lipopolysaccharide; positive regulation of leukocyte chemotaxis; glucose transport; leukocyte migration during inflammatory response; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; platelet degranulation; positive regulation of cell division; defense response to bacterium; immune response; blood coagulation; inflammatory response

NCBI Summary:The protein encoded by this gene is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. It has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells. [provided by RefSeq, May 2010]
UniProt Code:P02775
NCBI GenInfo Identifier:129874
NCBI Gene ID:5473
NCBI Accession:P02775.3
UniProt Secondary Accession:P02775,Q6IBJ8, B2R5F3,
UniProt Related Accession:P02775,AAB46877
Molecular Weight:13,894 Da
NCBI Full Name:Platelet basic protein
NCBI Synonym Full Names:pro-platelet basic protein (chemokine (C-X-C motif) ligand 7)
NCBI Official Symbol:PPBP  
NCBI Official Synonym Symbols:PBP; TC1; TC2; TGB; LDGF; MDGF; TGB1; B-TG1; CTAP3; CXCL7; NAP-2; SCYB7; THBGB; LA-PF4; THBGB1; Beta-TG; CTAPIII; CTAP-III  
NCBI Protein Information:platelet basic protein; thrombocidin 1; thrombocidin 2; beta-thromboglobulin; CXC chemokine ligand 7; C-X-C motif chemokine 7; thromboglobulin, beta-1; small inducible cytokine B7; small-inducible cytokine B7; leukocyte-derived growth factor; low-affinity platelet factor IV; neutrophil-activating peptide 2; neutrophil-activating peptide-2; macrophage-derived growth factor; connective tissue-activating peptide III; small inducible cytokine subfamily B, member 7
UniProt Protein Name:Platelet basic protein
UniProt Synonym Protein Names:C-X-C motif chemokine 7; Leukocyte-derived growth factor; LDGF; Macrophage-derived growth factor; MDGF; Small-inducible cytokine B7Cleaved into the following 10 chains:Connective tissue-activating peptide III; CTAP-III; Alternative name(s):; LA-PF4; Low-affinity platelet factor IV
Protein Family:Placenta growth factor
UniProt Gene Name:PPBP  
UniProt Entry Name:CXCL7_HUMAN

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

Before adding to wells, equilibrate the SABC working solution and TMB substrate for at least 30 min at 37°C. When diluting samples and reagents, they must be mixed completely and evenly. It is recommended to plot a standard curve for each test.

StepProtocol
1.Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample in duplicate. Wash plate 2 times before adding standard, sample and control (zero) wells!
2.Aliquot 0.1ml standard solutions into the standard wells.
3.Add 0.1 ml of Sample / Standard dilution buffer into the control (zero) well.
4.Add 0.1 ml of properly diluted sample ( Human serum, plasma, tissue homogenates and other biological fluids.) into test sample wells.
5.Seal the plate with a cover and incubate at 37 °C for 90 min.
6.Remove the cover and discard the plate content, clap the plate on the absorbent filter papers or other absorbent material. Do NOT let the wells completely dry at any time. Wash plate X2.
7.Add 0.1 ml of Biotin- detection antibody working solution into the above wells (standard, test sample & zero wells). Add the solution at the bottom of each well without touching the side wall.
8.Seal the plate with a cover and incubate at 37°C for 60 min.
9.Remove the cover, and wash plate 3 times with Wash buffer. Let wash buffer rest in wells for 1 min between each wash.
10.Add 0.1 ml of SABC working solution into each well, cover the plate and incubate at 37°C for 30 min.
11.Remove the cover and wash plate 5 times with Wash buffer, and each time let the wash buffer stay in the wells for 1-2 min.
12.Add 90 µl of TMB substrate into each well, cover the plate and incubate at 37°C in dark within 10-20 min. (Note: This incubation time is for reference use only, the optimal time should be determined by end user.) And the shades of blue can be seen in the first 3-4 wells (with most concentrated standard solutions), the other wells show no obvious color.
13.Add 50 µl of Stop solution into each well and mix thoroughly. The color changes into yellow immediately.
14. Read the O.D. absorbance at 450 nm in a microplate reader immediately after adding the stop solution.

When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.

Sample TypeProtocol
SerumIf using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

PlasmaCollect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit.
Urine & Cerebrospinal FluidCollect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid.
Cell culture supernatantCollect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately.
Cell lysatesSolubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Tissue homogenatesThe preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C.
Tissue lysatesRinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Breast MilkCollect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles.

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