Human CXCL16 (Chemokine C-X-C-Motif Ligand 16) ELISA Kit
The CXCL16 ELISA Kit is a cutting-edge assay meticulously crafted for the precise quantification of Chemokine C-X-C-Motif Ligand 16 (CXCL16) levels in human biological samples. CXCL16, a key chemokine, plays a crucial role in immune responses, including regulation of T-cell migration and adhesion in inflammatory processes.
Accurate detection of CXCL16 levels is imperative for unraveling its involvement in immune regulation, inflammation, and disease pathogenesis. Monitoring CXCL16 using this specialized kit provides valuable insights for understanding immune responses and developing targeted therapeutic interventions. Our CXCL16 ELISA Kit guarantees exceptional sensitivity and specificity, ensuring highly reliable and reproducible results necessary for both research and clinical applications. Engineered under stringent quality control measures, this kit delivers robust performance and user-friendly operation, making it an ideal choice for your investigative and diagnostic needs. Place your trust in Assay Genie's CXCL16 ELISA Kit for precise and trustworthy quantification of this critical biomarker in your research endeavors.
Product Name:
Human CXCL16 (Chemokine C-X-C-Motif Ligand 16) ELISA Kit
SKU:
AEES00048
Target:
Human CXCL16 (Chemokine C-X-C-Motif Ligand 16)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CXCL16. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CXCL16 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CXCL16, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CXCL16. You can calculate the concentration of Human CXCL16 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-95
84-98
89-98
Average (%)
90
90
92
1:4
Range (%)
97-107
94-107
93-103
Average (%)
103
99
99
1:8
Range (%)
91-105
98-104
94-104
Average (%)
97
101
97
1:16
Range (%)
90-100
97-107
89-97
Average (%)
95
101
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
83-95
90
EDTA plasma (n=5)
88-100
93
Cell culture media (n=5)
89-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.83
2.45
8.69
0.93
2.27
9.12
Standard deviation
0.05
0.12
0.44
0.05
0.11
0.46
C V (%)
6.02
4.9
5.06
5.38
4.85
5.04
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CXCL16 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CXCL16 in samples. No significant cross-reactivity or interference between Human CXCL16 and analogues was observed.
