The Human CUBN (Cubilin) ELISA Kit is specifically designed for the precise measurement of cubilin levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results that are perfect for various research applications.Cubilin is a key protein that plays a vital role in the uptake of various nutrients, including vitamin B12 and protein-protein interactions.
Dysregulation of cubilin has been linked to conditions such as metabolic disorders and kidney diseases, highlighting its importance as a biomarker for studying these diseases and potential therapeutic interventions.With its user-friendly design and robust performance, the Human CUBN (Cubilin) ELISA Kit is an invaluable tool for researchers looking to delve deeper into the role of cubilin in health and disease.
Product Name:
Human CUBN (Cubilin) ELISA Kit
SKU:
HUES03327
Target:
Human CUBN (Cubilin) ELISA Kit
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CUBN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CUBN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CUBN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CUBN. You can calculate the concentration of Human CUBN in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-101
85-97
92-109
Average (%)
92
92
100
1:4
Range (%)
90-103
79-93
88-102
Average (%)
97
85
95
1:8
Range (%)
92-104
86-101
83-97
Average (%)
97
93
90
1:16
Range (%)
93-105
84-97
83-97
Average (%)
98
90
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-106
101
EDTA plasma (n=5)
88-100
93
Cell culture media (n=5)
95-108
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.35
5.2
17.41
2.41
4.7
18.84
Standard deviation
0.16
0.28
0.87
0.16
0.27
0.69
C V (%)
6.81
5.38
5.0
6.64
5.74
3.66
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CUBN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CUBN in samples. No significant cross-reactivity or interference between Human CUBN and analogues was observed.
