The Human CTSC (Cathepsin C) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of Cathepsin C levels in human samples. Cathepsin C is a lysosomal protease that plays a key role in the activation of granule-associated serine proteases in immune cells, making it a crucial player in the immune response.This kit offers reliable and reproducible results, making it ideal for researchers studying conditions related to the immune system, such as autoimmune diseases, infections, and inflammatory disorders.
By measuring Cathepsin C levels, researchers can gain valuable insights into the immune response and potentially identify new therapeutic targets for these conditions.Overall, the Human CTSC (Cathepsin C) CLIA Kit is a valuable tool for researchers looking to study the role of Cathepsin C in immune function and its implications for various diseases.
Product Name:
Human CTSC (Cathepsin C) CLIA Kit
SKU:
HUES00113
Target:
Human CTSC (Cathepsin C)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CTSC. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CTSC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CTSC, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CTSC. You can calculate the concentration of Human CTSC in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-106
87-100
87-101
Average (%)
100
92
93
1:4
Range (%)
101-118
85-97
86-101
Average (%)
109
90
93
1:8
Range (%)
100-114
90-106
98-112
Average (%)
107
97
103
1:16
Range (%)
101-116
83-95
89-104
Average (%)
108
90
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
93
EDTA plasma (n=5)
84-97
91
Cell culture media (n=5)
94-108
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
269.21
663.95
2234.25
245.86
726.13
2401.14
Standard deviation
21.73
50.26
161.54
31.13
72.76
231.23
C V (%)
8.07
7.57
7.23
12.66
10.02
9.63
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CTSC concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CTSC in samples. No significant cross-reactivity or interference between Human CTSC and analogues was observed.
