The Human CTNN (Beta-Catenin) CLIA Kit is specifically designed for the precise measurement of beta-catenin levels in human samples such as serum, plasma, and cell culture supernatants. This kit is characterized by its exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Beta-catenin is a key protein involved in cell-cell adhesion and gene expression regulation, playing a crucial role in various cellular processes such as cell proliferation and differentiation.
Dysregulation of beta-catenin has been implicated in diseases such as cancer, developmental disorders, and inflammatory conditions, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Human CTNN (Beta-Catenin) CLIA Kit provides researchers with a reliable tool for investigating the role of beta-catenin in health and disease, offering insights that can contribute to advancements in medical research and drug development.
Product Name:
Human CTNN beta1 (Catenin, Beta 1) CLIA Kit
SKU:
HUES00439
Target:
Human CTNN beta1 (Catenin, Beta 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CTNNβ1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CTNNβ1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CTNNβ1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CTNNβ1. You can calculate the concentration of Human CTNNβ1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-111
87-98
88-101
Average (%)
104
93
95
1:4
Range (%)
96-110
87-98
100-114
Average (%)
103
93
107
1:8
Range (%)
84-96
95-112
92-108
Average (%)
91
103
99
1:16
Range (%)
96-113
95-108
93-103
Average (%)
103
102
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
98
EDTA plasma (n=5)
92-107
99
Cell culture media (n=5)
88-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
99.73
229.73
837.66
109.03
209.57
774.89
Standard deviation
12.85
23.2
86.87
8.84
23.05
63.39
C V (%)
12.88
10.1
10.37
8.11
11.0
8.18
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CTNNβ1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CTNNβ1 in samples. No significant cross-reactivity or interference between Human CTNNβ1 and analogues was observed.
