Human CTDSP1 Recombinant Protein (RPPB1508)
- SKU:
- RPPB1508
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q9GZU7
- Research Area:
- Enzymes
Description
| Product Name: | Human CTDSP1 Recombinant Protein |
| Product Code: | RPPB1508 |
| Size: | 10µg |
| Species: | Human |
| Target: | CTDSP1 |
| Synonyms: | Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1, Nuclear LIM interactor-interacting factor 3, NLI-IF, NLI-interacting factor 3, Small C-terminal domain phosphatase 1, SCP1, Small CTD phosphatase 1, CTDSP1, NIF3, NLIIF. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | CTDSP1 solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 20% glycerol and 0.1M NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MDSSAVITQI SKEEARGPLR GKGDQKSAAS QKPRSRGILH SLFCCVCRDD GEALPAHSGA PLLVEENGAI PKTPVQYLLP EAKAQDSDKI CVVIDLDETL VHSSFKPVNN ADFIIPVEID GVVHQVYVLK RPHVDEFLQR MGELFECVLF TASLAKYADP VADLLDKWGA FRARLFRESC VFHRGNYVKD LSRLGRDLRR VLILDNSPAS YVFHPDNAVP VASWFDNMSD TELHDLLPFF EQLSRVDDVY SVLRQPRPGS |
CTDSP1 is a class 2C phosphatase with activity dependent on the conserved DxD motif. CTDSP1 preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. In addition, CTDSP1 negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation.
CTDSP1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 280 amino acids (1-260 a.a.) and having a molecular mass of 31.2kDa.CTDSP1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CTDSP1: Preferentially catalyzes the dephosphorylation of 'Ser- 5' within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 3.1.3.16; Protein phosphatase, Ser/Thr (non-receptor) Chromosomal Location of Human Ortholog: 2q35 Cellular Component: nucleus Molecular Function:protein binding; metal ion binding; CTD phosphatase activity Biological Process: regulation of transcription from RNA polymerase II promoter; negative regulation of neuron differentiation; negative regulation of protein amino acid phosphorylation; protein amino acid dephosphorylation |
| NCBI Summary: | This gene encodes a member of the small C-terminal domain phosphatase (SCP) family of nuclear phosphatases. These proteins play a role in transcriptional regulation through specific dephosphorylation of phosphoserine 5 within tandem heptapeptide repeats of the C-terminal domain of RNA polymerase II. The encoded protein plays a role in neuronal gene silencing in non-neuronal cells, and may also inhibit osteoblast differentiation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2011] |
| UniProt Code: | Q9GZU7 |
| NCBI GenInfo Identifier: | 17865510 |
| NCBI Gene ID: | 58190 |
| NCBI Accession: | Q9GZU7.1 |
| UniProt Secondary Accession: | Q9GZU7,Q7Z5Q3, Q7Z5Q4, C9IYG0, |
| UniProt Related Accession: | Q9GZU7 |
| Molecular Weight: | 29,128 Da |
| NCBI Full Name: | Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1 |
| NCBI Synonym Full Names: | CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 1 |
| NCBI Official Symbol: | CTDSP1�� |
| NCBI Official Synonym Symbols: | NIF3; SCP1; NLIIF; NLI-IF�� |
| NCBI Protein Information: | carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1; NLI-interacting factor 3; small C-terminal domain phosphatase 1; nuclear LIM interactor-interacting factor 3 |
| UniProt Protein Name: | Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1 |
| UniProt Synonym Protein Names: | Nuclear LIM interactor-interacting factor 3; NLI-IF; NLI-interacting factor 3; Small C-terminal domain phosphatase 1; SCP1; Small CTD phosphatase 1 |
| Protein Family: | Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase |
| UniProt Gene Name: | CTDSP1�� |
| UniProt Entry Name: | CTDS1_HUMAN |
