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Human CTDSP1 Recombinant Protein (RPPB1508)

SKU:
RPPB1508
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9GZU7
Research Area:
Enzymes
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Description

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Product Name:Human CTDSP1 Recombinant Protein
Product Code:RPPB1508
Size:10µg
Species:Human
Target:CTDSP1
Synonyms:Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1, Nuclear LIM interactor-interacting factor 3, NLI-IF, NLI-interacting factor 3, Small C-terminal domain phosphatase 1, SCP1, Small CTD phosphatase 1, CTDSP1, NIF3, NLIIF.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:CTDSP1 solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 20% glycerol and 0.1M NaCl.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MDSSAVITQI SKEEARGPLR GKGDQKSAAS QKPRSRGILH SLFCCVCRDD GEALPAHSGA PLLVEENGAI PKTPVQYLLP EAKAQDSDKI CVVIDLDETL VHSSFKPVNN ADFIIPVEID GVVHQVYVLK RPHVDEFLQR MGELFECVLF TASLAKYADP VADLLDKWGA FRARLFRESC VFHRGNYVKD LSRLGRDLRR VLILDNSPAS YVFHPDNAVP VASWFDNMSD TELHDLLPFF EQLSRVDDVY SVLRQPRPGS

CTDSP1 is a class 2C phosphatase with activity dependent on the conserved DxD motif. CTDSP1 preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. In addition, CTDSP1 negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation.

CTDSP1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 280 amino acids (1-260 a.a.) and having a molecular mass of 31.2kDa.CTDSP1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:CTDSP1: Preferentially catalyzes the dephosphorylation of 'Ser- 5' within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 3.1.3.16; Protein phosphatase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: nucleus

Molecular Function:protein binding; metal ion binding; CTD phosphatase activity

Biological Process: regulation of transcription from RNA polymerase II promoter; negative regulation of neuron differentiation; negative regulation of protein amino acid phosphorylation; protein amino acid dephosphorylation

NCBI Summary:This gene encodes a member of the small C-terminal domain phosphatase (SCP) family of nuclear phosphatases. These proteins play a role in transcriptional regulation through specific dephosphorylation of phosphoserine 5 within tandem heptapeptide repeats of the C-terminal domain of RNA polymerase II. The encoded protein plays a role in neuronal gene silencing in non-neuronal cells, and may also inhibit osteoblast differentiation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2011]
UniProt Code:Q9GZU7
NCBI GenInfo Identifier:17865510
NCBI Gene ID:58190
NCBI Accession:Q9GZU7.1
UniProt Secondary Accession:Q9GZU7,Q7Z5Q3, Q7Z5Q4, C9IYG0,
UniProt Related Accession:Q9GZU7
Molecular Weight:29,128 Da
NCBI Full Name:Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1
NCBI Synonym Full Names:CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 1
NCBI Official Symbol:CTDSP1��
NCBI Official Synonym Symbols:NIF3; SCP1; NLIIF; NLI-IF��
NCBI Protein Information:carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1; NLI-interacting factor 3; small C-terminal domain phosphatase 1; nuclear LIM interactor-interacting factor 3
UniProt Protein Name:Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1
UniProt Synonym Protein Names:Nuclear LIM interactor-interacting factor 3; NLI-IF; NLI-interacting factor 3; Small C-terminal domain phosphatase 1; SCP1; Small CTD phosphatase 1
Protein Family:Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase
UniProt Gene Name:CTDSP1��
UniProt Entry Name:CTDS1_HUMAN

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