Human CST6 Recombinant Protein (RPPB3293)
- SKU:
- RPPB3293
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q15828
Description
| Product Name: | Human CST6 Recombinant Protein |
| Product Code: | RPPB3293 |
| Size: | 10µg |
| Species: | Human |
| Target: | CST6 |
| Synonyms: | Cystatin E/M, cystatin 6, Cystatin M, Cystatin-E, Cysteine proteinase inhibitor. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The CST6 solution (0.5mg/1ml) contains 10% glycerol, 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MRPQERMVGE LRDLSPDDPQ VQKAAQAAVA SYNMGSNSIY YFRDTHIIKA QSQLVAGIKY FLTMEMGSTD CRKTRVTGDH VDLTTCPLAA GAQQEKLRCD FEVLVVPWQN SSQLLKHNCV QM |
| Biological Activity: | The IC50 value is < 10nM. The inhibitory function of Cystatin 6 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25?C. |
Cystatin E/M or CST6 is part of the cystatin type 2 family. Part of the cystatin type 2 family members can act as cysteine protease inhibitors, whereas cystatin E/M regulates cathepsin B inhibitors and not cathepsin C. cystatin E/M is a protein the when secreted, has an effect on osteogenesis and bone resorption, insulin regulation, response to systemic inflammation & hepatocyte growth factor receptors.
CST6 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 142 amino acids (29-149 a.a.) and having a molecular mass of 15.9kDa.CST6 is fused to a 21 amino acid His tag at N-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CST6: Shows moderate inhibition of cathepsin B but is not active against cathepsin C. Belongs to the cystatin family. |
| UniProt Protein Details: | Protein type:Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide Chromosomal Location of Human Ortholog: 11q13 Cellular Component: cornified envelope Molecular Function:cysteine protease inhibitor activity Biological Process: epidermis development; anatomical structure morphogenesis |
| NCBI Summary: | The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. This gene encodes a cystatin from the type 2 family, which is down-regulated in metastatic breast tumor cells as compared to primary tumor cells. Loss of expression is likely associated with the progression of a primary tumor to a metastatic phenotype. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q15828 |
| NCBI GenInfo Identifier: | 4503113 |
| NCBI Gene ID: | 1474 |
| NCBI Accession: | NP_001314.1 |
| UniProt Secondary Accession: | Q15828,Q540N7, |
| UniProt Related Accession: | Q15828 |
| Molecular Weight: | 16,511 Da |
| NCBI Full Name: | cystatin-M |
| NCBI Synonym Full Names: | cystatin E/M |
| NCBI Official Symbol: | CST6�� |
| NCBI Protein Information: | cystatin-M; cystatin 6; cystatin M; cystatin-6; cystatin-E; cystatin M/E; cysteine proteinase inhibitor |
| UniProt Protein Name: | Cystatin-M |
| UniProt Synonym Protein Names: | Cystatin-6; Cystatin-E |
| Protein Family: | Cystatin |
| UniProt Gene Name: | CST6�� |
| UniProt Entry Name: | CYTM_HUMAN |
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