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Human CST5 Recombinant Protein (RPPB3291)

SKU:
RPPB3291
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P28325
€309
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Description

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Product Name:Human CST5 Recombinant Protein
Product Code:RPPB3291
Size:10µg
Species:Human
Target:CST5
Synonyms:Cystatin-D, CST5, Cystatin 5, Cystatin-5.
Source:Sf9 Insect cells
Physical Appearance:Sterile Filtered clear solution.
Formulation:The CST5 solution (0.5mg/1ml) contains phosphate buffered saline (pH7.4) and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:QSRTLAGGIH ATDLNDKSVQ RALDFAISEY NKVINKDEYY SRPLQVMAAY QQIVGGVNYY FNVKFGRTTC TKSQPNLDNC PFNDQPKLKE EEFCSFQINE VPWEDKISIL NYKCRKVHHH HHH
Biological Activity:The IC50 value is < 2.0nM. The inhibitory function of Cystatin 5 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.

Cystatin 5 (CST5) is a novel member of the cystatin superfamily, which encompasses proteins that contain multiple cystatin-like sequences. Some members are active cysteine protease inhibitors, while others have lost or possibly never had this inhibitory activity. The superfamily is comprised of 3 inhibitory families: the type 1 cystatins (stefins), type 2 cystatins and the kininogens. Type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in various human fluids and secretions.

CST5 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 123 amino acids (26-142 a.a) and having a molecular mass of 14.36kDa. CST5 is fused to a 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:CST5: Cysteine proteinase inhibitor that possibly plays a protective role against proteinases present in the oral cavity. The order of preference for inhibition is cathepsin S > cathepsin H > cathepsin L > cathepsin B. Belongs to the cystatin family.Protein type: Secreted, signal peptide; SecretedChromosomal Location of Human Ortholog: 20p11.21Cellular Component: extracellular regionMolecular Function: protein binding; cysteine protease inhibitor activity
UniProt Protein Details:
NCBI Summary:The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a protein found in saliva and tears. The encoded protein may play a protective role against proteinases present in the oral cavity. [provided by RefSeq, Jul 2008]
UniProt Code:P28325
NCBI GenInfo Identifier:19882256
NCBI Gene ID:1473
NCBI Accession:NP_001891.2
UniProt Secondary Accession:P28325,Q5JRF5, Q9UCA0
UniProt Related Accession:P28325
Molecular Weight:16,080 Da
NCBI Full Name:cystatin-D
NCBI Synonym Full Names:cystatin D
NCBI Official Symbol:CST5��
NCBI Official Synonym Symbols:
NCBI Protein Information:cystatin-D; cystatin 5; cystatin-5; cysteine-proteinase inhibitor
UniProt Protein Name:Cystatin-D
UniProt Synonym Protein Names:Cystatin-5
Protein Family:Cystatin
UniProt Gene Name:CST5��
UniProt Entry Name:CYTD_HUMAN

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