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Human Crystallin Alpha B (CRYaB) ELISA Kit

SKU:
HUDL00693
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P02511
Sensitivity:
0.216ng/mL
Range:
0.625-40ng/mL
ELISA Type:
Sandwich
Synonyms:
CRY-AB,CRYA-B,CRYA2,CTPP2,HSPB5,Heat shock protein beta-5,Renal carcinoma antigen NY-REN-27,Rosenthal fiber component
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Crystallin Alpha B (CRYaB) ELISA Kit

The Human Crystallin Alpha B (CRYAB) ELISA Kit is specifically designed for the accurate measurement of CRYAB levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and consistent results for a variety of research purposes.CRYAB, also known as alpha B-crystallin, is a key protein involved in maintaining cellular structure and function, particularly in the eye lens and muscle tissues. Abnormal levels of CRYAB have been linked to various diseases such as cataracts, myopathies, and neurodegenerative disorders, highlighting its importance as a potential biomarker for disease diagnosis and therapeutic development.

With its reliable performance and versatility, the Human Crystallin Alpha B (CRYAB) ELISA Kit is a valuable tool for researchers studying the role of CRYAB in health and disease, paving the way for new insights and treatment strategies.

Product Name:Human Crystallin Alpha B (CRYaB) ELISA Kit
Product Code:HUDL00693
Size:96 Assays
Target:CRYaB
Synonyms:CRY-AB, CRYA-B, CRYA2, CTPP2, HSPB5, Heat shock protein beta-5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component
Detection Method:Sandwich
Range:0.625-40ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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