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Human CRYM Recombinant Protein (RPPB3273)

SKU:
RPPB3273
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q14894
$328
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Description

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Product Name:Human CRYM Recombinant Protein
Product Code:RPPB3273
Size:10µg
Species:Human
Target:CRYM
Synonyms:Crystallin Mu, Thiomorpholine-Carboxylate Dehydrogenase, THBP, NADP-Regulated Thyroid-Hormone Binding Protein, NADP-Regulated Thyroid-Hormone-Binding Protein, Mu-Crystallin Homolog, EC 1.5.1.25, DFNA40, CRYM.
Source:Escherichia Coli
Physical Appearance:Filtered White lyophilized (freeze-dried) powder.
Formulation:CRYM was filtered (0.4�m) and lyophilized from 0.5mg/ml in 20mM Tris buffer and 50mM NaCl, pH 7.5.
Solubility:It is recommended to add 200�l of deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. CRYM is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Stability:Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MKHHHHHHAS RVPAFLSAAE VEEHLRSSSL LIPPLETALA NFSSGPEGGV MQPVRTVVPV TKHRGYLGVM PAYSAAEDAL TTKLVTFYED RGITSVVPSH QATVLLFEPS NGTLLAVMDG NVITAKRTAA VSAIATKFLK PPSSEVLCIL GAGVQAYSHY EIFTEQFSFK EVRIWNRTKE NAEKFADTVQ GEVRVCSSVQ EAVAGADVII TVTLATEPIL FGEWVKPGAH INAVGASRPD WRELDDELMK EAVLYVDSQE AALKESGDVL LSGAEIFAEL GEVIKGVKPA HCEKTTVFKS LGMAVEDTVA AKLIYDSWSS GK

Crystallin, Mu (CRYM) is a taxon-specific crystallin protein which binds NADPH and has sequence similarity to bacterial ornithine cyclodeaminases. CRYM doesn�t perform a structural role in lens tissue; instead CRYM binds thyroid hormone for possible regulatory or developmental roles. CRYM gene mutations are linked with autosomal dominant non-syndromic deafness. CRYM specifically catalyzes the reduction of imine bonds in brain substrates which may include cystathionine ketamine and lanthionine ketamine.

CRYM Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (Ser2-Lys314) containing 322 amino acids including a 9 aa His tag at N-terminus. The total calculated molecular mass is 34.8kDa.

UniProt Protein Function:CRYM: Specifically catalyzes the reduction of imine bonds in brain substrates that may include cystathionine ketimine (CysK) and lanthionine ketimine (LK). Binds thyroid hormone which is a strong reversible inhibitor. Presumably involved in the regulation of the free intracellular concentration of triiodothyronine and access to its nuclear receptors. Belongs to the ornithine cyclodeaminase family.
UniProt Protein Details:

Protein type:Lyase; EC 1.5.1.25

Chromosomal Location of Human Ortholog: 16p12.2

Cellular Component: mitochondrion; cytoplasm; nucleus

Molecular Function:thiomorpholine-carboxylate dehydrogenase activity; protein homodimerization activity; NADP binding; transcription corepressor activity

Biological Process: sensory perception of sound; negative regulation of transcription from RNA polymerase II promoter; thyroid hormone metabolic process

Disease: Deafness, Autosomal Dominant 40

NCBI Summary:Crystallins are separated into two classes: taxon-specific and ubiquitous. The former class is also called phylogenetically-restricted crystallins. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. This gene encodes a taxon-specific crystallin protein that binds NADPH and has sequence similarity to bacterial ornithine cyclodeaminases. The encoded protein does not perform a structural role in lens tissue, and instead it binds thyroid hormone for possible regulatory or developmental roles. Mutations in this gene have been associated with autosomal dominant non-syndromic deafness. [provided by RefSeq, Sep 2014]
UniProt Code:Q14894
NCBI GenInfo Identifier:2498259
NCBI Gene ID:1428
NCBI Accession:Q14894.1
UniProt Related Accession:Q14894
Molecular Weight:36kDa
NCBI Full Name:Ketimine reductase mu-crystallin
NCBI Synonym Full Names:crystallin mu
NCBI Official Symbol:CRYM��
NCBI Official Synonym Symbols:THBP; DFNA40��
NCBI Protein Information:ketimine reductase mu-crystallin
UniProt Protein Name:Ketimine reductase mu-crystallin
UniProt Synonym Protein Names:NADP-regulated thyroid-hormone-binding protein
Protein Family:Ketimine reductase
UniProt Gene Name:CRYM��
UniProt Entry Name:CRYM_HUMAN

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