The Human CRY1 (Cryptochrome 1) CLIA Kit is specifically designed for the precise measurement of CRY1 levels in human biological samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers consistent and dependable results, making it well-suited for various research applications.CRY1 is a key protein involved in the regulation of circadian rhythms, acting as a core component of the circadian clock. Dysfunction in CRY1 has been linked to various health conditions including sleep disorders, metabolic diseases, and mood disorders.
Therefore, accurate measurement of CRY1 levels with this kit is essential for gaining insights into these conditions and exploring potential therapeutic interventions.Overall, the Human CRY1 CLIA Kit from Assay Genie provides researchers with a reliable tool for studying the role of CRY1 in circadian biology and its implications in various health disorders.
Product Name:
Human CRY1 (Cryptochrome 1) CLIA Kit
SKU:
HUES01181
Target:
Human CRY1 (Cryptochrome 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CRY1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CRY1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CRY1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CRY1. You can calculate the concentration of Human CRY1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-117
94-111
100-115
Average (%)
108
102
107
1:4
Range (%)
95-110
93-105
85-99
Average (%)
102
100
91
1:8
Range (%)
97-112
89-103
87-101
Average (%)
103
96
95
1:16
Range (%)
94-109
94-107
86-102
Average (%)
101
101
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-114
105
EDTA plasma (n=5)
89-101
94
Cell culture media (n=5)
97-114
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
197.24
373.59
1618.13
204.52
358.69
1504.24
Standard deviation
23.61
43.07
181.07
22.11
39.78
109.81
C V (%)
11.97
11.53
11.19
10.81
11.09
7.3
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CRY1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CRY1 in samples. No significant cross-reactivity or interference between Human CRY1 and analogues was observed.
