Human CRH (Corticotropin Releasing Hormone) ELISA Kit
The Human CRH (Corticotropin-Releasing Hormone) ELISA Kit is specifically designed for the precise measurement of CRH levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers unparalleled sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.CRH is a vital hormone that plays a key role in the body's stress response system, regulating various physiological processes including metabolism, immune response, and behavior.
Abnormal CRH levels have been linked to conditions such as anxiety disorders, depression, and chronic stress-related diseases, making it a valuable biomarker for studying these disorders and exploring potential treatment options.Overall, the Human CRH ELISA Kit provides researchers with a reliable tool for investigating the role of CRH in human health and disease, paving the way for new discoveries in the field of neuroendocrinology.
Product Name:
Human CRH (Corticotropin Releasing Hormone) ELISA Kit
SKU:
HUES02230
Target:
Human CRH (Corticotropin Releasing Hormone)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
2.35 pg/mL
Detection range:
3.91-250 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with CRH. During the reaction, CRH in the sample or standard competes with a fixed amount of CRH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to CRH. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CRH in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-104
91-105
94-110
Average (%)
96
98
101
1:4
Range (%)
87-100
91-104
95-110
Average (%)
93
97
103
1:8
Range (%)
87-100
93-107
95-113
Average (%)
95
99
103
1:16
Range (%)
87-98
92-106
94-107
Average (%)
92
100
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-107
100
EDTA plasma (n=5)
94-109
100
Cell culture media (n=5)
94-105
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.1
5.9
17.2
2.2
5.3
16.3
Standard deviation
0.1
0.3
0.7
0.1
0.2
0.5
C V (%)
4.76
5.08
4.07
4.55
3.77
3.07
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of CRH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes CRH in samples. No significant cross-reactivity or interference between CRH and analogues was observed.
