The Human Creatine Transporter (CRT) ELISA Kit offered by AssayGenie is a powerful tool for measuring creatine transporter levels in human samples. This kit is specifically designed for accurate detection of CRT in serum, plasma, and cell culture supernatants, providing researchers with precise and reliable results.Creatine transporter is essential for the transport of creatine into cells, where it plays a crucial role in energy metabolism and muscle function. Dysregulation of CRT has been associated with various health conditions, including muscular dystrophy, kidney disorders, and neurological diseases.
By studying CRT levels, researchers can gain valuable insights into these conditions and potentially identify new therapeutic avenues.With high sensitivity and specificity, the Human CRT ELISA Kit from AssayGenie is an indispensable tool for any research focused on creatine transporter biology. Trust AssayGenie for accurate and reproducible results in your studies of CRT and its implications for human health.
Product Name:
Human Creatine Transporter (CRT) ELISA Kit
Product Code:
HUDL00691
Size:
96 Assays
Target:
CRTR
Synonyms:
SLC6A8, CT1, CRTR, Sodium And Chloride Dependent Creatine Transporter 1, Solute Carrier Family 6 Member 8
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
