Human Creatine Kinase, Mitochondrial 1A (CKMT1A) ELISA Kit
The Human Creatine Kinase Mitochondrial 1A (CKMT1A) ELISA Kit is a powerful tool for researchers looking to accurately measure levels of CKMT1A in human serum, plasma, and cell culture supernatants. With excellent sensitivity and specificity, this kit delivers reliable and reproducible results for a variety of research applications.CKMT1A is an important enzyme involved in cellular energy production, specifically in the mitochondria. By measuring levels of CKMT1A, researchers can gain valuable insights into muscle metabolism and energy utilization.
This enzyme is essential for maintaining optimal muscle function, making it a key biomarker for studying conditions related to muscle health and energy metabolism.Whether studying muscle disorders, exercise physiology, or mitochondrial dysfunction, the Human CKMT1A ELISA Kit provides a reliable and accurate method for quantifying CKMT1A levels in human samples. Trust in this kit to support your research efforts and advance scientific understanding in the field of muscle biology.
Product Name:
Human Creatine Kinase, Mitochondrial 1A (CKMT1A) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
