The Ceruloplasmin (CP) ELISA Kit is meticulously developed for the precise and quantitative determination of ceruloplasmin levels in human samples.
Ceruloplasmin, a critical copper-binding protein, serves vital roles in iron metabolism and antioxidant defense mechanisms. Monitoring ceruloplasmin levels is crucial for assessing iron homeostasis, copper transport, and overall oxidative balance in the body. Accurate measurement of ceruloplasmin helps in understanding its implications in various metabolic processes and clinical conditions. Our CP ELISA Kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for the quantification of ceruloplasmin in diverse biological samples. With stringent quality control measures in place, this kit guarantees robust performance and straightforward protocols, making it an optimal choice for both research and clinical applications. Rely on Assay Genie's CP ELISA Kit for reliable and precise quantification of this essential biomarker, facilitating insightful studies in your research endeavors.
Product Name:
Human CP (Ceruloplasmin) ELISA Kit
SKU:
AEES00062
Target:
Human CP (Ceruloplasmin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 μg/mL
Detection range:
0.78-50 μg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CP. You can calculate the concentration of Human CP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-108
86-102
98-106
Average (%)
101
94
103
1:4
Range (%)
96-110
90-98
92-103
Average (%)
103
94
96
1:8
Range (%)
94-109
94-104
92-106
Average (%)
101
98
99
1:16
Range (%)
89-100
96-102
91-99
Average (%)
96
99
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-101
95
EDTA plasma (n=5)
90-102
96
Cell culture media (n=5)
85-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.02
5.9
21.85
2.23
5.36
20.31
Standard deviation
0.12
0.29
1.11
0.11
0.26
0.87
C V (%)
5.94
4.92
5.08
4.93
4.85
4.28
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CP in samples. No significant cross-reactivity or interference between Human CP and analogues was observed.
