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Human Cortisol ELISA Kit (AEES00016)

SKU:
AEES00016
Product Type:
ELISA Kit
ELISA Type:
Competitive
Size:
96 Assays
Reactivity:
Human
Sensitivity:
2.92 ng/mL
Range:
6.25-400 ng/mL
Sample Type:
Serum
€499
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Human Cortisol ELISA Kit
SKU:AEES00016
Target:Human Cortisol
Size:96T
Assay type:Competitive-ELISA
Assay time:2.0h
Sensitivity:2.92 ng/mL
Detection range:6.25-400 ng/mL
Kit component:
ComponentQuantity (24 Assays)Quantity (96 Assays)Storage
Micro ELISA Plate (Dismountable)8 wells x 3 strips8 wells x 12 strips-20°C, 6 months
Reference Standard1 vial2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 60 µL1 vial, 120 µL
Concentrated HRP Conjugate (100×)1 vial, 60 µL1 vial, 120 µL-20°C (shading light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL1 vial, 20 mL4°C, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL1 vial, 30 mL
Substrate Reagent1 vial, 10 mL1 vial, 10 mL4°C (shading light)
Stop Solution1 vial, 10 mL1 vial, 10 mL4°C
Plate Sealer5 pieces5 pieces
Product Description1 copy1 copy

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Cortisol. During the reaction, Cortisol in the sample or standard competes with a fixed amount of Cortisol on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Cortisol. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cortisol in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Linearity:
Serum (n=5)EDTA plasma (n=5)Cell culture media (n=5)
1:2Range (%)80-9088-10285-107
Average (%)859394
1:4Range (%)81-9687-9580-101
Average (%)889092
1:8Range (%)84-10282-8983-95
Average (%)958686
1:16Range (%)87-10489-10484-99
Average (%)939988
Recovery:
Sample TypeRange (%)Average Recovery (%)
Serum (n=5)81-9385
EDTA plasma (n=5)86-9689
Cell culture media (n=5)85-10095
Precision:
Intra-assay PrecisionInter-assay Precision
Sample123123
n2020.020.020.020.020.0
Mean (ng/mL)50.33100.32200.5251.2799.84202.18
Standard deviation4.428.0814.94.597.0214.46
C V (%)8.788.057.438.967.037.15
Sample type &Sample volume:serum, plasma, urine; 50μL
Reproducibility:Both intra-CV and inter-CV are < 10%.
Application:This ELISA kit applies to the in vitro quantitative determination of Cortisol concentrations in serum, plasma, urine.
Specificity:This kit recognizes Cortisol in samples. No significant cross-reactivity or interference between Cortisol and analogues was observed.

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