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Human Corticotropin Releasing Hormone Receptor 2 (CRHR2) ELISA Kit

SKU:
HUDL00676
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q13324
Sensitivity:
0.051ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CRFR2,CRF2,Corticotropin-releasing factor receptor 2
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
$779
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Description

system_update_altDatasheet

Human Corticotropin Releasing Hormone Receptor 2 (CRHR2) ELISA Kit

The Human Corticotropin-Releasing Hormone Receptor 2 (CRHR2) ELISA Kit is specifically designed for the precise measurement of CRHR2 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers outstanding sensitivity and accuracy, ensuring dependable and consistent results for a variety of research purposes.CRHR2 is a critical receptor involved in the stress response system, playing a key role in regulating stress-induced behaviors and physiological responses.

Dysregulation of CRHR2 has been linked to various psychiatric disorders, making it a valuable target for studying stress-related conditions and potential therapeutic interventions.With its advanced technology and reliable performance, the Human CRHR2 ELISA Kit is an essential tool for researchers studying stress biology, psychiatric disorders, and drug development. Trust in this kit to deliver precise and reproducible data for your research needs.

Product Name:Human Corticotropin Releasing Hormone Receptor 2 (CRHR2) ELISA Kit
Product Code:HUDL00676
Size:96 Assays
Target:CRHR2
Synonyms:CRFR2, CRF2, Corticotropin-releasing factor receptor 2
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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